PMC:4829102 / 31392-32007 JSONTXT

{"project":"2_test","denotations":[{"id":"26807646-17965729-73828208","span":{"begin":91,"end":93},"obj":"17965729"},{"id":"26807646-17965729-73828208","span":{"begin":91,"end":93},"obj":"17965729"}],"text":"Homologous recombination DR-GFP assay\n48 hours after siRNA transfection, U2OS DR-GFP cells 16 were co-transfected using Amaxa nucleofection with an I-SceI expression vector (pCMV-I-SceI) and a vector expressing mCherry fluorescent protein (pmCherry-C1). 24 hours after I-SceI transfection cells were harvested and analysed by flow cytometry (CyAn ADP Analyzer, Beckman Coulter). The percentage of GFP-positive cells among transfected cells (mCherry-positive cells) was determined using Summit 4.3 software. siControl treated sample was set as 100%. Statistical significance was determined with the Student’s t-test."}