PMC:4786408 / 12629-13359 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4786408","sourcedb":"PMC","sourceid":"4786408","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4786408","text":"10.7554/eLife.12245.006Figure 4. E122D mutation interferes with formation of the ATG12–ATG5 conjugate.\n(A) Coomassie Blue-stained SDS-PAGE gel following glutathione affinity purification from lysates of Hi5 cells infected with baculoviruses expressing GST-ATG12 and either WT or E122D mutant ATG5. (B and C) HEK293 cells expressing the indicated proteins were analyzed by IB. (D) Drosophila whole bodies expressing the indicated transgenes under the control of Tub-Gal4 were analyzed by IB. (E) Lysates from Hi5 cells expressing the indicated proteins were subjected to His/Ni-NTA purification and subsequent TEV protease treatment. Proteins were analyzed by Coomassie Blue staining.\nDOI: http://dx.doi.org/10.7554/eLife.12245.006","divisions":[{"label":"Title","span":{"begin":33,"end":102}}],"tracks":[]}