PMC:4718983 / 7043-7594 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4718983","sourcedb":"PMC","sourceid":"4718983","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4718983","text":"Synaptic blockers\nAll experiments were conducted on cultured cortical neurons that were functionally isolated from their network by a pharmacological block of glutamatergic and GABAergic synapses. For each culture 20 μl of a cocktail of synaptic blockers was used, consisting of 10 μM CNQX (6-cyano-7-nitroquinoxaline-2,3-dione), 80 μM APV (amino-5-phosphonovaleric acid) and 5 μM bicuculline. This cocktail did not block the spontaneous network activity completely, but rather made it sparse. At least 1 h was allowed for stabilization of the effect.","divisions":[{"label":"title","span":{"begin":0,"end":17}}],"tracks":[]}