PMC:4701709 / 7483-8380 JSONTXT

{"project":"2_test","denotations":[{"id":"28330081-17582919-320","span":{"begin":172,"end":176},"obj":"17582919"},{"id":"28330081-18453006-321","span":{"begin":298,"end":302},"obj":"18453006"}],"text":"Sedative activity (open field test)\nThe sedative effect of the crude extract was evaluated according to published procedures using the open field method (Walsh and Cummins 1976). This method has been proven effective in the assessment of locomotor activity and anxiety-linked behaviors (Lau et al. 2008). The apparatus used for the test consisted of a white wooden area (150 cm in diameter) enclosed in stainless steel walls and the area was divided by black lines, into four squares. The open field was placed inside a light and sound-attenuated room. The mice were adapted to red light (40 W) for 60 min prior to the start of experiment and fed ad libitum. The animals were administered with normal saline and Z. nummularia root extract at the doses of 50 and 100 mg/kg. After 30 min of the treatment, each mouse was placed in the center of the box and the numbers of lines crossed were counted."}