PMC:4608092 / 7509-8596 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4608092","sourcedb":"PMC","sourceid":"4608092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4608092","text":"Evaluation of Angiogenic Activity by Tube Formation and Migration Assays\nTube formation was assessed as described previously.14,21 HUVECs (30 000 cells/well; 0 or 1 ng/mL of IFN-β) were seeded onto 96-well plates coated with Matrigel (BD Biosciences, San Jose, CA). After incubation for 20 hours at 37°C, digital images of the tubes that formed were captured. For quantification, the area of the tube, total length of the tube, and number of tube branches were calculated using ImageJ software (National Institutes of Health, Bethesda, MD).\nMigration assays were performed as described previously.22 HUVECs were grown to overconfluence in 24-well plates and then incubated overnight for serum starvation (0 or 1 ng/mL of IFN-β). After creation of a wound with a p200 pipette tip, the medium was replaced with endothelial growth medium-2 (0 or 1 ng/mL of IFN-β). The wound was allowed to narrow for healing (re-endothelialization) for 8 hours. Digital images were obtained before and after the incubation period, and the area of re-endothelialization was calculated using ImageJ software.","divisions":[{"label":"Title","span":{"begin":0,"end":72}}],"tracks":[{"project":"2_test","denotations":[{"id":"26126547-23850618-55294620","span":{"begin":125,"end":127},"obj":"23850618"},{"id":"26126547-17872458-55294621","span":{"begin":128,"end":130},"obj":"17872458"},{"id":"26126547-20488443-55294622","span":{"begin":597,"end":599},"obj":"20488443"}],"attributes":[{"subj":"26126547-23850618-55294620","pred":"source","obj":"2_test"},{"subj":"26126547-17872458-55294621","pred":"source","obj":"2_test"},{"subj":"26126547-20488443-55294622","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#a2ec93","default":true}]}]}}