PMC:4608092 / 42248-43456 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4608092","sourcedb":"PMC","sourceid":"4608092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4608092","text":"Figure 16 ATPase activity of RNF213 WT, R4810K, and ΔAAA mutants. A, Lysates from EGFP-RNF213–transfected HEK293 cells were IP with anti-GFP agarose. A total volume of 15 μL was subjected to SDS-PAGE followed by GelCode staining. RNF213 proteins fused with EGFP were detected in EGFP-RNF213–transfected cells (arrow heads, “EGFP-RNF213”). Representative SDS-PAGE images are shown. Similar results were obtained in 3 independent experiments. B, ATPase activity of immunoprecipitated extracts was assayed for ATPase activity. Indicated volumes (μL) of IP products were combined with buffer to yield a total volume of 50 μL for ATPase reaction for 30 minutes at room temperature (see details in the text). Phosphate release was measured using the Phosphate Sensor as ATPase activity. Relative activity was calculated based on average activity of EGFP at 0 μL, which was equal to 1. Data with bars represent mean±SD (n=3). *P\u003c0.05 according to Student t test compared with WT at 2 volumes. EGFP indicates enhanced green fluorescent proteins detected in EGFP-transfected cells (arrow head); IgG HC, IgG heavy chain; IgG LC, IgG light chain; IP, immunoprecipitated; Well, sample wells of the gel; WT, wild type.\n\n","tracks":[]}