PMC:4608092 / 39936-41376 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4608092","sourcedb":"PMC","sourceid":"4608092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4608092","text":"Figure 15 Angiogenic activity of RNF213 WT, and R4810K, WEQ, and ΔAAA mutants. A, RNF213 protein expression in HUVECs transiently expressing the RNF213 mutant. HUVECs transfected with the FLAG-RNF213 expression vector (RNF213 WT, RNF213 R4810K, RNF213 WEQ, and RNF213 ΔAAA) were immunoblotted using anti-FLAG antibodies. Empty vector (“vector” in the figure)-transfected HUVECs served as the control. Representative western blotting findings are shown. Similar results were obtained in 3 independent experiments. B, Tube formation assays for HUVECs transiently expressing RNF213 WT, RNF213 R4810K, RNF213 WEQ, and RNF213 ΔAAA. The vector served as the positive control (100%). Scale bars indicate 100 μm. Representative images are shown in left panel. Tube area was quantified by imaging analysis (right panel). A column with a bar represents mean±SD (n=3). *P\u003c0.05, according to Student t test compared with the vector. C, Migration assays for HUVECs transiently expressing RNF213 WT, RNF213 R4810K, RNF213 WEQ, and RNF213 ΔAAA. The vector served as the control. Scale bars indicate 100 μm. Representative images are shown in left panel. Re-endothelialized areas were quantified by imaging analysis (right panel). A column with a bar represents mean±SD (n=3). *P\u003c0.05 according to Student t test compared with the vector. HUVECs indicates human umbilical vein endothelial cells; LPF, low-pass filter; WEQ, Walker B motif; WT, wild-type. ","tracks":[]}