PMC:4608092 / 33539-35314
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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4608092","sourcedb":"PMC","sourceid":"4608092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4608092","text":"Figure 11 Effects of RNF213 depletion on antiangiogenic activity of IFN-β in HUVECs. A, Western blot analysis of RNF213 protein expression in HUVECs treated with IFN-β for 24 hours after control or RNF213 siRNA transfection. β-tubulin served as the loading control. Representative western blotting results are shown in left panel. A column with a bar (right panel) represents mean±SD (n=3). *P\u003c0.05, according to Student t test comparing 0 ng/mL of IFN-β within the same siRNA treatment paradigm. #P\u003c0.05, cells exposed to 10 ng/mL of IFN-β were compared between RNF213 siRNA treatment and no treatment using Student t test. †P\u003c0.05, using Student t test comparing control siRNA at 10 ng/mL of IFN-β. B, Tube formation assays for HUVECs cultured with IFN-β on Matrigel after control or RNF213 siRNA transfection. Treatment with 0 ng/mL of IFN-β after control siRNA transfection was used as a positive control (100%). Scale bars indicate 100 μm. Representative images are shown in left panel. Tube area was quantified by imaging analysis (right panel). A column with a bar represents mean±SD (n=3). *P\u003c0.05, according to Student t test comparing 0 ng/mL of IFN-β within the same siRNA treatment paradigm. C, Migration assays for HUVECs treated with IFN-β (1 ng/mL) after control or RNF213 siRNA transfection. Treatment with 0 ng/mL of IFN-β after control siRNA transfection served as the control. Scale bars indicate 100 μm. Representative images are shown in left panel. Re-endothelialized areas were quantified by imaging analysis (right panel). A column with a bar represents mean±SD (n=3). *P\u003c0.05, according to Student t test comparing 0 ng/mL of IFN-β within the same siRNA treatment paradigm. HUVECs indicates human umbilical vein endothelial cells; IFN, interferon.\nF","tracks":[]}