PMC:4608092 / 32010-33540 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4608092","sourcedb":"PMC","sourceid":"4608092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4608092","text":"Figure 10 Effects of STAT1 depletion on RNF213 expression and antiangiogenic activities of IFN-β in HUVECs. A, Western blot analyses of RNF213 and STAT1 protein expressions and STAT1 protein phosphorylation (at Ser727; p-STAT1) in HUVECs treated with IFN-β for 24 hours after control or STAT1 siRNA transfection. β-tubulin served as the loading control. Representative western blot experiments are shown in the upper panel. A column with a bar (lower panel) represents mean±SD (n=3). *P\u003c0.05 according to Student t test compared with 0 ng/mL of IFN-β with the same siRNA treatment. #P\u003c0.05, cells treated with 10 ng/mL of IFN-β were compared between STAT1 siRNA treatment and no siRNA treatment using Student t test. †P\u003c0.05, STAT1 siRNA treatment was compared with control siRNA at 10 ng/mL of IFN-β using Student t test. B, Tube formation assays for HUVECs cultured with IFN-β on Matrigel after control or STAT1 siRNA transfection. Treatment with 0 ng/mL of IFN-β after control siRNA transfection was evaluated as a positive control (100%). Scale bars indicate 100 μm. Representative images are shown in upper panel. The tube area, total tube length, and number of branches were quantified by imaging analysis (lower panel). A column with a bar represents mean±SD (n=3). *P\u003c0.05, according to Student t test comparing 0 ng/mL of IFN-β within the same siRNA treatment paradigm. HUVECs indicates human umbilical vein endothelial cells; IFN, interferon; p-STAT1, phosphorylated signal transducer and activator of transduction 1.\nF","tracks":[]}