PMC:4608092 / 12583-13417 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4608092","sourcedb":"PMC","sourceid":"4608092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4608092","text":"Biochemical Assays\nReal-time quantitative PCR (qPCR) and western blotting were used as biochemical assays to assess mRNA and protein expression, respectively. qPCR was performed as described previously.8 Target cDNA expression levels were normalized to corresponding expression levels of PPIA. The primer pairs that were used were as described previously.8,14\nWestern blotting was performed as described previously.15 Lysates were subjected to immunoblotting using an anti-RNF213 antibody that we previously generated14 or anti-STAT1 (sc-346; Santa Cruz Biotechnology), anti-FLAG (NU01102; Nacalai Tesque), anti-phospho-STAT1 (Ser727; #8826; Cell Signaling Technology, Beverly, MA), and anti-β-tubulin (sc-9104; Santa Cruz Biotechnology) antibodies. Quantification was conducted using Scion Image software (Scion Corp, Frederick, MD).","divisions":[{"label":"Title","span":{"begin":0,"end":18}}],"tracks":[{"project":"2_test","denotations":[{"id":"26126547-21799892-55294626","span":{"begin":202,"end":203},"obj":"21799892"},{"id":"26126547-21799892-55294627","span":{"begin":355,"end":356},"obj":"21799892"},{"id":"26126547-23850618-55294628","span":{"begin":357,"end":359},"obj":"23850618"},{"id":"26126547-23994138-55294629","span":{"begin":415,"end":417},"obj":"23994138"},{"id":"26126547-23850618-55294630","span":{"begin":517,"end":519},"obj":"23850618"}],"attributes":[{"subj":"26126547-21799892-55294626","pred":"source","obj":"2_test"},{"subj":"26126547-21799892-55294627","pred":"source","obj":"2_test"},{"subj":"26126547-23850618-55294628","pred":"source","obj":"2_test"},{"subj":"26126547-23994138-55294629","pred":"source","obj":"2_test"},{"subj":"26126547-23850618-55294630","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#939bec","default":true}]}]}}