PMC:4576194 / 31684-32187 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4576194","sourcedb":"PMC","sourceid":"4576194","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4576194","text":"4.10. Real-Time RT-PCR\nTotal RNA was extracted from the culture supernatant of CSFV-infected PK-15 cells using a TRIzol reagent (catalog no. 15596026; Invitrogen, Waltham, MA, USA). The isolated RNA was then reverse transcribed to cDNA with reverse transcriptase XL (AMV) (catalog no. 2621; TaKaRa, Dalian, China) according to the manufacturer’s instructions. Genomic copies of CSFV were quantified by a real-time RT-PCR assay as described previously [25]. The experiment was carried out in triplicates.","divisions":[{"label":"Title","span":{"begin":0,"end":22}}],"tracks":[{"project":"2_test","denotations":[{"id":"26266418-17658704-144197563","span":{"begin":452,"end":454},"obj":"17658704"}],"attributes":[{"subj":"26266418-17658704-144197563","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93b9ec","default":true}]}]}}