PMC:4576194 / 25649-26265 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4576194","sourcedb":"PMC","sourceid":"4576194","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4576194","text":"4.4. Plasmid Transfection\nHEK293T cells grown in 6-well plates (Nunc, Waltham, MA, USA) were cotransfected with 5 µg of pMyc-eEF1A and 3 µg of p3×Flag-NS5A using an X-tremeGENE HP DNA transfection reagent (catalog no. 06366236001; Roche, Penzberg, Germany) according to the manufacturer’s instructions. Briefly, the cells were transfected with 400 µL of serum-free DMEM containing 8 µg of plasmids and 12 µL of X-tremeGENE HP DNA transfection reagent. At 6 h post-transfection (hpt), the transfection mixture was replaced with DMEM supplemented with 10% FBS and incubated for an additional 48 h before being assayed.","divisions":[{"label":"Title","span":{"begin":0,"end":25}}],"tracks":[]}