PMC:4576194 / 23637-24245 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4576194","sourcedb":"PMC","sourceid":"4576194","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4576194","text":"4.2. Construction of Expression Vectors\nThe swine eEF1A gene (GenBank accession no. NM_001097418.2) and its various domains were amplified by PCR and cloned into the pMyc vector (Clontech, Palo Alto, CA, USA) to generate pMyc-eEF1A, pMyc-eEF1A(1-333), pMyc-eEF1A(1-237), pMyc-eEF1A(238-462) and pCMV-Myc-eEF1A(201-333), respectively. The CSFV NS5A gene (GenBank accession no. EU497410.1) was amplified by PCR and cloned into the p3×Flag-CMV-10 vector (Sigma-Aldrich, ST. Louis, MO, USA) or pCMV-Myc to generate p3×Flag-NS5A or pMyc-NS5A. The primers for amplifying eEF1A and NS5A genes are listed in Table 1.","divisions":[{"label":"Title","span":{"begin":0,"end":39}}],"tracks":[]}