PMC:4576194 / 10562-11406 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4576194","sourcedb":"PMC","sourceid":"4576194","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4576194","text":"2.3. Overexpression of eEF1A Inhibits CSFV Replication\nTo investigate the effects of eEF1A on the CSFV replication, stable PK-15 cell line overexpressing EGFP-eEF1A (PK-EGFP-eEF1A) or EGFP (PK-EGFP) was generated and infected with the CSFV Shimen strain at a multiplicity of infection (MOI) of 0.1. The Npro expression in the CSFV-infected PK-EGFP-eEF1A cells was decreased at 48 and 72 h post-infection (hpi), compared with that in the PK-EGFP cells (Figure 3A,B). Real-time RT-PCR analysis revealed that the viral genome copies in the PK-EGFP-eEF1A cells were also reduced at 48 and 72 hpi, compared with the PK-EGFP cells (Figure 3C). Additionally, yields of infectious virus in culture supernatant of PK-EGFP-eEF1A cells were decreased at 48 and 72 hpi (Figure 3D). The results indicated the eEF1A negatively modulates the CSFV replication.","divisions":[{"label":"Title","span":{"begin":0,"end":54}}],"tracks":[]}