PMC:4573257 / 19041-19733 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4573257","sourcedb":"PMC","sourceid":"4573257","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4573257","text":"In order to further corroborate a direct role of TRMT5 in mt-tRNA modification, we downregulated its amount by using RNA interference in HeLa cells (Figure 4D)36 and confirmed the reduction of TRMT5 amounts by western blotting (Figure S4B). The siRNA-mediated depletion of TRMT5 resulted in G37 hypomodification in mt-tRNALeu(CUN) in the RT-PEx assay, as observed for the samples derived from the affected individuals (Figure 4D). Taken together, our results thus far establish TRMT5 as the methyltransferase responsible for m1G37 modification in human mitochondrial tRNAs and suggest the variants detected in subjects 65205 and 73901 as responsible for their respiratory-chain abnormalities.","tracks":[{"project":"2_test","denotations":[{"id":"26189817-24986917-2053346","span":{"begin":159,"end":161},"obj":"24986917"}],"attributes":[{"subj":"26189817-24986917-2053346","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#cdec93","default":true}]}]}}