PMC:4504148 / 29462-31940 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4504148","sourcedb":"PMC","sourceid":"4504148","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4504148","text":"Induction of ER-related processes upon mutDFNA5 expression in yeast\nIn addition to the comparison of mutDFNA5 and wtDFNA5 in the post-diauxic shift, the modifications between mid-exponential phase and at the post-diauxic shift were investigated separately both in mutDFNA5-(comparison 2a Figure 1) and wtDFNA5-(comparison 2b Figure 1) transformed yeast cells. As both wtDFNA5 and mutDFNA5 cells demonstrated differentially expressed genes in exponential phase compared to post-diauxic shift, we expected the presence of many significantly up- or down regulated genes related to the post-diauxic shift but not solely due to mutDFNA5 expression. Therefore, genes which were differentially expressed at the post-diauxic shift upon mutDFNA5 transformation, but do not show any differences upon wtDFNA5 expression in post-diauxic phase, are potentially related to mutDFNA5-associated processes. These were assigned as mutDFNA5-related changes not associated with the post-diauxic shift in yeast.\nGenes with an adjusted p-value below 0.05 and a log2 (fold change) above 1.5 were selected for GO analysis. The GO-enriched processes significantly associated with up-regulated genes were very similar between comparison 2a and 2b and were associated with translation. GO-enriched terms significantly associated with down-regulated genes were related to ribosomes and RNA and were present both in wtDFNA5- and in mutDFNA5-transformed yeast cells. These processes were probably due to the shift to respiration and not in particular related to mutDFNA5 (data not shown). However, three main classes could be distinguished at the post-diauxic shift. Two of them were more prominent in mutDFNA5-transformed yeast cells. One class was related to the biosynthesis and the metabolism of lipids (GO:0008610), such as (ergo)sterols (GO:0016126), (phyto)steroids (GO:0006694), and fatty acids (GO:0006633) (Supplemental Data Table 4, indicated in bold). The other group was associated with the ER (GO:0005783), such as the ER membrane (GO:0005789) and protein targeting to ER (GO: 0045047) (Supplemental Data Table 4, processes are underlined).\nThe third group which could be distinguished was related to the cytoskeleton (GO:0005856) and was more pronounced in wtDFNA5-transformed yeast cells. Cellularly enriched GO terms such as the microtubule cytoskeleton (GO:0015630) and the microtubule organizing center (GO:0005815) were present in this list (Supplemental Data Table 5, indicated in bold).","divisions":[{"label":"Title","span":{"begin":0,"end":67}}],"tracks":[]}