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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4504005","sourcedb":"PMC","sourceid":"4504005","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4504005","text":"Plasmid construction, mutagenesis, protein expression, purification, and the AlphaScreen peptide-binding assay were as previously described with minor modifications to the AlphaScreen assay (Hill and Pioszak, 2013; Moad and Pioszak, 2013). Synthetic peptides were from RS Synthesis, Bachem, or were synthesized in-house. Details are in Supplemental Experimental Procedures.","tracks":[{"project":"TEST0","denotations":[{"id":"25982113-209-217-8319056","span":{"begin":209,"end":213},"obj":"[\"23247088\", \"23247088\", \"23247088\"]"},{"id":"25982113-233-241-8319057","span":{"begin":233,"end":237},"obj":"[\"24115156\", \"24115156\", \"24115156\"]"}],"attributes":[{"subj":"25982113-209-217-8319056","pred":"source","obj":"TEST0"},{"subj":"25982113-233-241-8319057","pred":"source","obj":"TEST0"}]},{"project":"2_test","denotations":[{"id":"25982113-23247088-118216157","span":{"begin":209,"end":213},"obj":"23247088"},{"id":"25982113-23247088-118216157","span":{"begin":209,"end":213},"obj":"23247088"},{"id":"25982113-24115156-71076136","span":{"begin":233,"end":237},"obj":"24115156"},{"id":"25982113-24115156-71076136","span":{"begin":233,"end":237},"obj":"24115156"},{"id":"25982113-23247088-71076136","span":{"begin":233,"end":237},"obj":"23247088"},{"id":"25982113-23247088-71076136","span":{"begin":233,"end":237},"obj":"23247088"},{"id":"T78983","span":{"begin":233,"end":237},"obj":"24115156"},{"id":"T7621","span":{"begin":233,"end":237},"obj":"24115156"}],"attributes":[{"subj":"25982113-23247088-118216157","pred":"source","obj":"2_test"},{"subj":"25982113-23247088-118216157","pred":"source","obj":"2_test"},{"subj":"25982113-24115156-71076136","pred":"source","obj":"2_test"},{"subj":"25982113-24115156-71076136","pred":"source","obj":"2_test"},{"subj":"25982113-23247088-71076136","pred":"source","obj":"2_test"},{"subj":"25982113-23247088-71076136","pred":"source","obj":"2_test"},{"subj":"T78983","pred":"source","obj":"2_test"},{"subj":"T7621","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#ec9395","default":true},{"id":"2_test","color":"#93afec"}]}]}}