PMC:4504005 / 33339-33765 JSONTXT

{"project":"TEST0","denotations":[{"id":"25982113-209-217-8319056","span":{"begin":262,"end":266},"obj":"[\"23247088\", \"23247088\", \"23247088\"]"},{"id":"25982113-233-241-8319057","span":{"begin":286,"end":290},"obj":"[\"24115156\", \"24115156\", \"24115156\"]"}],"text":"Protein Production and Characterization and Peptides\nPlasmid construction, mutagenesis, protein expression, purification, and the AlphaScreen peptide-binding assay were as previously described with minor modifications to the AlphaScreen assay (Hill and Pioszak, 2013; Moad and Pioszak, 2013). Synthetic peptides were from RS Synthesis, Bachem, or were synthesized in-house. Details are in Supplemental Experimental Procedures."}

{"project":"2_test","denotations":[{"id":"25982113-23247088-118216157","span":{"begin":262,"end":266},"obj":"23247088"},{"id":"25982113-23247088-118216157","span":{"begin":262,"end":266},"obj":"23247088"},{"id":"25982113-24115156-71076136","span":{"begin":286,"end":290},"obj":"24115156"},{"id":"25982113-24115156-71076136","span":{"begin":286,"end":290},"obj":"24115156"},{"id":"25982113-23247088-71076136","span":{"begin":286,"end":290},"obj":"23247088"},{"id":"25982113-23247088-71076136","span":{"begin":286,"end":290},"obj":"23247088"},{"id":"T78983","span":{"begin":286,"end":290},"obj":"24115156"},{"id":"T7621","span":{"begin":286,"end":290},"obj":"24115156"}],"text":"Protein Production and Characterization and Peptides\nPlasmid construction, mutagenesis, protein expression, purification, and the AlphaScreen peptide-binding assay were as previously described with minor modifications to the AlphaScreen assay (Hill and Pioszak, 2013; Moad and Pioszak, 2013). Synthetic peptides were from RS Synthesis, Bachem, or were synthesized in-house. Details are in Supplemental Experimental Procedures."}