PMC:4504005 / 29228-29928 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4504005","sourcedb":"PMC","sourceid":"4504005","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4504005","text":"Distinct RAMP binding site augmentation clearly contributes to peptide selectivity (Figure 6). RAMP2 E101 favors AM binding because it can hydrogen bond with Y52 and RAMP2 F111 discourages CGRP binding because it is too small to contact the F37 phenyl ring. Indeed, the F37Y swap in CGRPmut conferred strong affinity for the AM1 receptor ECD complex and the Y52F swap in AM(37-52)NH2 significantly diminished its binding. The lack of Glu at RAMP1 position 74 would disfavor strong AM binding. RAMP1 W84 enables strong CGRP binding by contacting F37, but this contact alone is apparently insufficient for selectivity because AM(37-52)NH2 [Y52F] did not gain affinity for the CGRP receptor ECD complex.","tracks":[]}