PMC:4504005 / 26281-27267
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4504005","sourcedb":"PMC","sourceid":"4504005","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4504005","text":"The CGRPmut and AM binding modes are consistent with peptide mutagenesis studies. CGRP T30, V32, and F37 and the C-terminal amide were important for binding purified CLR:RAMP1 ECD (Moad and Pioszak, 2013) and intact CGRP receptor (Carpenter et al., 2001; Rist et al., 1998; Watkins et al., 2013b). Modified CGRP peptides as short as 30–37 maintained the ability to bind the receptor (Carpenter et al., 2001), consistent with this region providing most of the contacts. Increased affinity of CGRPmut over that of CGRP can be explained by their differences in the turn region (Figure 1D). P34 favors β-turn formation better than S34 and F35 provides better hydrophobic contact to CLR loop 4 than K35. AM P43, K46, I47, G51, Y52, and the C-terminal amide were most critical for binding purified CLR:RAMP2 ECD and intact AM1 receptor, and truncation beyond residue 38 diminished binding even though the K38-V41 side chains were not important (Moad and Pioszak, 2013; Watkins et al., 2013a).","tracks":[{"project":"TEST0","denotations":[{"id":"25982113-117-125-8319041","span":{"begin":199,"end":203},"obj":"[\"24115156\", \"24115156\", \"24115156\"]"},{"id":"25982113-167-175-8319042","span":{"begin":249,"end":253},"obj":"[\"11444978\", \"11444978\", \"11444978\"]"},{"id":"25982113-186-194-8319043","span":{"begin":268,"end":272},"obj":"[\"9438028\", \"9438028\", \"9438028\"]"},{"id":"25982113-104-112-8319045","span":{"begin":402,"end":406},"obj":"[\"11444978\", \"11444978\", \"11444978\"]"},{"id":"25982113-235-243-8319046","span":{"begin":957,"end":961},"obj":"[\"24115156\", \"24115156\", \"24115156\"]"}],"attributes":[{"subj":"25982113-117-125-8319041","pred":"source","obj":"TEST0"},{"subj":"25982113-167-175-8319042","pred":"source","obj":"TEST0"},{"subj":"25982113-186-194-8319043","pred":"source","obj":"TEST0"},{"subj":"25982113-104-112-8319045","pred":"source","obj":"TEST0"},{"subj":"25982113-235-243-8319046","pred":"source","obj":"TEST0"}]},{"project":"2_test","denotations":[{"id":"25982113-24115156-118216142","span":{"begin":199,"end":203},"obj":"24115156"},{"id":"25982113-24115156-118216142","span":{"begin":199,"end":203},"obj":"24115156"},{"id":"25982113-11444978-118216143","span":{"begin":249,"end":253},"obj":"11444978"},{"id":"25982113-11444978-118216143","span":{"begin":249,"end":253},"obj":"11444978"},{"id":"25982113-9438028-118216144","span":{"begin":268,"end":272},"obj":"9438028"},{"id":"25982113-9438028-118216144","span":{"begin":268,"end":272},"obj":"9438028"},{"id":"25982113-11444978-118216146","span":{"begin":402,"end":406},"obj":"11444978"},{"id":"25982113-11444978-118216146","span":{"begin":402,"end":406},"obj":"11444978"},{"id":"25982113-24115156-118216147","span":{"begin":957,"end":961},"obj":"24115156"},{"id":"25982113-24115156-118216147","span":{"begin":957,"end":961},"obj":"24115156"},{"id":"25982113-24115156-71076128","span":{"begin":199,"end":203},"obj":"24115156"},{"id":"25982113-24115156-71076128","span":{"begin":199,"end":203},"obj":"24115156"},{"id":"25982113-11444978-71076130","span":{"begin":402,"end":406},"obj":"11444978"},{"id":"25982113-11444978-71076130","span":{"begin":402,"end":406},"obj":"11444978"}],"attributes":[{"subj":"25982113-24115156-118216142","pred":"source","obj":"2_test"},{"subj":"25982113-24115156-118216142","pred":"source","obj":"2_test"},{"subj":"25982113-11444978-118216143","pred":"source","obj":"2_test"},{"subj":"25982113-11444978-118216143","pred":"source","obj":"2_test"},{"subj":"25982113-9438028-118216144","pred":"source","obj":"2_test"},{"subj":"25982113-9438028-118216144","pred":"source","obj":"2_test"},{"subj":"25982113-11444978-118216146","pred":"source","obj":"2_test"},{"subj":"25982113-11444978-118216146","pred":"source","obj":"2_test"},{"subj":"25982113-24115156-118216147","pred":"source","obj":"2_test"},{"subj":"25982113-24115156-118216147","pred":"source","obj":"2_test"},{"subj":"25982113-24115156-71076128","pred":"source","obj":"2_test"},{"subj":"25982113-24115156-71076128","pred":"source","obj":"2_test"},{"subj":"25982113-11444978-71076130","pred":"source","obj":"2_test"},{"subj":"25982113-11444978-71076130","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#93beec","default":true},{"id":"2_test","color":"#d8ec93"}]}]}}