PMC:4504005 / 16758-17680 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4504005","sourcedb":"PMC","sourceid":"4504005","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4504005","text":"Superposition of the AM-bound and ligand-free CLR:RAMP2 complexes (Kusano et al., 2012) revealed minor CLR conformational differences involving the N-terminal region of α1 moving toward AM in the AM-bound state presumably due to AM-CLR T37 contacts (Figure 3F). CLR loops 3 and 4 do not move as in the CGRPmut-bound structure and there are no significant side-chain conformational differences at the peptide-binding site. The position of RAMP2 relative to CLR varies in the two structures (Figure 3F), but the ligand-free RAMP2 position is probably constrained by formation of the dimer of heterodimers in which the C-terminal end of RAMP2 α2 occupies the peptide-binding site of CLR from the opposing heterodimer (Figure 3G). Dimerization of the CLR:RAMP2 ECD heterodimer thus occludes the AM-binding site, which suggested that the RAMP2 L106R substitution (at the end of RAMP2 α2) was key to obtaining AM-bound crystals.","tracks":[{"project":"TEST0","denotations":[{"id":"25982113-82-90-8319022","span":{"begin":82,"end":86},"obj":"[\"22102369\", \"22102369\", \"22102369\"]"}],"attributes":[{"subj":"25982113-82-90-8319022","pred":"source","obj":"TEST0"}]},{"project":"2_test","denotations":[{"id":"25982113-22102369-118216124","span":{"begin":82,"end":86},"obj":"22102369"},{"id":"25982113-22102369-118216124","span":{"begin":82,"end":86},"obj":"22102369"},{"id":"25982113-22102369-71076119","span":{"begin":82,"end":86},"obj":"22102369"},{"id":"25982113-22102369-71076119","span":{"begin":82,"end":86},"obj":"22102369"}],"attributes":[{"subj":"25982113-22102369-118216124","pred":"source","obj":"2_test"},{"subj":"25982113-22102369-118216124","pred":"source","obj":"2_test"},{"subj":"25982113-22102369-71076119","pred":"source","obj":"2_test"},{"subj":"25982113-22102369-71076119","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#d9ec93","default":true},{"id":"2_test","color":"#e493ec"}]}]}}