PMC:4502374 / 31625-33803
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502374","sourcedb":"PMC","sourceid":"4502374","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502374","text":"For QTL mapping, we used a progeny derived from a cross between the most likely B genome ancestor of cultivated peanut A. ipaënsis K 30076 (susceptible to rust), and the wild species A. magna K 30097 (resistant to rust). Arachis ipaënsis is very closely related to the B genome of cultivated peanut and has only ever been identified and collected from a single site in the wild. Arachis magna is very closely related to it (Krapovickas and Gregory 2007; Bechara et al. 2010; Moretzsohn et al. 2013) and may even be considered the same biological species (J. Valls, personal communication). We have used the progeny from this cross in two previously published studies. In the first, an F2 progeny was used to produce a map with 149 markers in 10 LGs, spanning a total of 1294 cM (Moretzsohn et al. 2009). In the second, a higher density genetic map of 798 loci, but covering only 461 cM, was constructed using F6 RILs generated by single seed descent. This map was integrated into a consensus framework of the separate diploid A and B genome components and tetraploid peanut (Shirasawa et al. 2013). Here, we used the same F6 lines and genotyping information from some additional markers to develop a map consisting of 399 high quality markers, none of which cosegregated. This map covered 678 cM. The differences in sizes of these maps, generated from the same parents, seem to be best explained by the different type of software used and parameters that treat genotyping errors in different ways (Bertioli et al. 2014). The largest size (1294 cM) was obtained with Mapmaker without error detection, the intermediate size (678 cM) was obtained by Mapmaker with error detection (Lincoln et al. 1992), and the smallest (461 cM) was obtained with Joinmap, a program that contains its own algorithms that detect error and correct genetic distances. Although it seems clear that the F2 genetic map distance of 1294 cM must be substantially inflated, it is unclear which of the other two estimates is better. Nevertheless, the map presented here has markers positioned with very high confidence, and, because it contains no cosegregating markers, it is better suited to QTL detection.","tracks":[{"project":"2_test","denotations":[{"id":"25943521-21092103-43367136","span":{"begin":469,"end":473},"obj":"21092103"},{"id":"25943521-19351409-43367137","span":{"begin":797,"end":801},"obj":"19351409"},{"id":"25943521-23315685-43367138","span":{"begin":1092,"end":1096},"obj":"23315685"},{"id":"25943521-24212082-43367139","span":{"begin":1514,"end":1518},"obj":"24212082"}],"attributes":[{"subj":"25943521-21092103-43367136","pred":"source","obj":"2_test"},{"subj":"25943521-19351409-43367137","pred":"source","obj":"2_test"},{"subj":"25943521-23315685-43367138","pred":"source","obj":"2_test"},{"subj":"25943521-24212082-43367139","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ece9","default":true}]}]}}