PMC:4502374 / 23779-25132 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502374","sourcedb":"PMC","sourceid":"4502374","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502374","text":"All markers evaluated in this study were amplified by the use of heterologous primers. With the use of minimum LOD score of 6.0 and a maximum recombination fraction (θ) of 0.35, and after the exclusion of co-segregating markers, we found that 399 markers mapped onto 10 LGs, spanning a total map distance of 678.2 cM. These markers included 378 microsatellites and 21 transposon (miniature inverted-repeat transposable element) markers. LGs were numbered and oriented essentially according to Moretzsohn et al. (2009), but with B05 and B08 reoriented (“flipped”) to ensure forward compatibility with pseudomolecules produced by the Peanut Genome Sequencing Consortium (www.peanutbase.org; Genbank GCA_000816755.1). LGs ranged from 41.5 cM (with 35 markers) to 139.2 cM (55 markers), with an average distance of 1.7 cM between adjacent markers. A total of 91 (22.8%) of the 399 mapped markers deviated from the expected 1:1 ratio at P \u003c 0.05 level. Of these, 79 markers were skewed toward A. ipaënsis and only 12 markers toward A. magna. Most LGs have few distorted markers, and LG B05 and B08 had no distorted markers. In contrast, LG B02 and the upper portion of LG B04 were almost entirely composed of distorted markers. These two LGs and LG B06 were distorted toward A. ipaënsis alleles. Distorted markers at P \u003c 0.05 were identified by # (Figure 3).","tracks":[{"project":"2_test","denotations":[{"id":"25943521-19351409-43367135","span":{"begin":512,"end":516},"obj":"19351409"}],"attributes":[{"subj":"25943521-19351409-43367135","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#e193ec","default":true}]}]}}