PMC:4502374 / 19674-20850 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502374","sourcedb":"PMC","sourceid":"4502374","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502374","text":"KASP assays were performed with the following genotypes: the diploids A. ipaënsis K 30076, A. batizocoi K9484 and A. magna K 30097, the induced allotetraploids (A. magna K 30097 x A. stenosperma V15076)4x (here called MagSten) and (A. batizocoi K9484 × A. stenosperma V10309)4x (here called BatSten1) and six A. hypogaea cultivars (Tifrunner, Tifguard, GA-06G, NC3033, ICVG 88145, and SPTG_06). Reactions consisted of 2 μL of KASP 2X reaction mix, 0.055 μL of assay primer mix (12 mM of each allele-specific primer and 30 mM of common primer) and 20 ng of genomic DNA, in a 4-µL volume. A C1000 Thermal Cycler (Bio-Rad) was used with the following cycling conditions: 94° for 15 min, nine cycles of 94° for 20 sec, touchdown starting at 65° for 60 sec (decreasing 0.8° per cycle), 29 cycles of 94° for 20 sec, and 57° for 60 sec (http://www.cerealsdb.uk.net/cerealgenomics/CerealsDB/PDFs/KASP_SNP_Genotyping_Manual.pdf). To improve the results, a second KASP program was run as following: 9 cycles of 94° for 20 sec and 57° for 60 sec. Fluorescence was read by a The LightCycler 480 Instrument II (Roche Life Science) and analyzed using the LightCycler 480 Software (V.1.5.1).","tracks":[]}