PMC:4502374 / 18387-19105 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502374","sourcedb":"PMC","sourceid":"4502374","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502374","text":"Allele-specific forward primers and a common reverse primer were designed for use in KASP assays (LGC Genomics Ltd. Hoddesdon, UK; http://www.lgcgenomics.com/kasp-genotyping-reagents) using BatchPrimer3 (http://probes.pw.usda.gov/batchprimer3/) with the “Allele specific primers and allele flanking primers” option. The parameters used were 60−120 bp in size, Tm between 58 and 60°, and GC content between 30 and 80%. The alternative alleles were marked with 6-FAM and reference alleles with VIC. For each SNP, two allele-specific forward primers and one common reverse primer were designed. A schematic diagram of SNP discovery and primer design is shown in Figure 1, A and B. Primer information is listed in File S1.","tracks":[]}