PMC:4502374 / 10695-12157
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502374","sourcedb":"PMC","sourceid":"4502374","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502374","text":"This newly developed framework map was used for QTL analysis. Phenotyping data included the components of resistance to P. arachidis and agronomic traits (Supporting Information, File S1). Traits phenotyped in different trials or bioassays were analyzed separately. The normality of data distribution was evaluated by skewness and kurtosis values using WinQTL Cartographer, version 2.5 (Wang et al. 2006). QTL were mapped by using the composite interval mapping method proposed by Zeng (1993, 1994) and also the WinQTL Cartographer. This software assumes that the quantitative data under analysis are normally distributed. Some of the data sets did not fit this assumption and were log(x) transformed. We performed composite interval mapping analysis with the standard model (Model 6), scanning the genetic map and estimating the likelihood of a QTL and its corresponding effects at every 1 cM while using eight significant marker cofactors to adjust the phenotypic effects associated with other positions in the genetic map. A window size of 10 cM was used, and therefore cofactors within 10 cM on either side of the QTL test site were not included in the QTL model. Thresholds were determined for each trait by permutation tests (Churchill and Doerge 1994; Doerge and Churchill 1996), by the use of 1000 permutations and a significance level of 0.05. Graphic presentation of the LGs and the significant QTL was drawn with MapChart, version 2.1 (Voorrips 2002).","tracks":[{"project":"2_test","denotations":[{"id":"25943521-8248199-43367128","span":{"begin":487,"end":491},"obj":"8248199"},{"id":"25943521-8013918-43367129","span":{"begin":493,"end":497},"obj":"8013918"},{"id":"25943521-7851788-43367130","span":{"begin":1253,"end":1257},"obj":"7851788"},{"id":"25943521-8770605-43367131","span":{"begin":1280,"end":1284},"obj":"8770605"},{"id":"25943521-12011185-43299416","span":{"begin":1456,"end":1460},"obj":"12011185"}],"attributes":[{"subj":"25943521-8248199-43367128","pred":"source","obj":"2_test"},{"subj":"25943521-8013918-43367129","pred":"source","obj":"2_test"},{"subj":"25943521-7851788-43367130","pred":"source","obj":"2_test"},{"subj":"25943521-8770605-43367131","pred":"source","obj":"2_test"},{"subj":"25943521-12011185-43299416","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93bb","default":true}]}]}}