PMC:4502372 / 20290-21316 JSONTXT

{"project":"2_test","denotations":[{"id":"25943524-20523895-43313284","span":{"begin":145,"end":149},"obj":"20523895"}],"text":"Loss of Sea3 impacts colony formation in the break-induced replication (BIR) assay strain and on bleomycin. (A) BIR assay strain (Lydeard et al. 2010). An HO cut site (HO), marked with HPH, is integrated into the CAN1 gene (represented as CA) on chromosome V, deleting the 3′ portion of CAN1. The CAN1 donor (represented as AN1), which shares 1157 bp of homology to the CAN1 gene, is integrated into chromosome XI. Sites marked with A indicate AvaI sites used for monitoring BIR repair in Figure 3B. (B) Platings for single colonies of wild-type and sea3Δ mutants in the BIR assay strain on YPD and YPGal. (C) Percent viability of wild-type and sea3Δ mutants as a ratio of number of colonies on YPGal divided by the dilution factor and then divided by the number of colonies on YPD. Values represent average of two independent trials and error bars indicate standard error of the mean (SEM). (D) Platings for single colonies of wild-type and sea3Δ mutants in the YPH274 genetic background on YPD and YPD + 3.5 μg/mL bleomycin."}