PMC:4502372 / 17627-18738
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502372","sourcedb":"PMC","sourceid":"4502372","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502372","text":"Two types of telomerase-independent survivors have been described, both of which rely on BIR to elongate telomeres in the absence of telomerase (Lundblad and Blackburn 1993; Lydeard et al. 2007; Teng and Zakian 1999). Type I survivors amplify subtelomeric Y´ elements and have short terminal telomeric repeat tracts, whereas Type II survivors amplify telomeric sequence from the very end of another telomere, resulting in highly heterogeneous lengths of terminal telomeric repeat tracts (Lundblad and Blackburn 1993; Teng and Zakian 1999). Type II survivors predominant in serial liquid culture assays because of a growth advantage over Type I survivors (Teng and Zakian 1999). We found that sea3∆ tlc1Δ mutants still formed Type II survivors in liquid culture, but the survivors took approximately 1−2 d longer to be visualized than for tlc1∆ mutants (Figure 1D), indicating a possible delay in the execution of BIR or in growth post survivor formation. Thus, although the absence of Sea3 did not impact normal telomere length maintenance, it did impact growth and recovery of telomerase-independent survivors.","tracks":[{"project":"2_test","denotations":[{"id":"25943524-8477448-43313277","span":{"begin":168,"end":172},"obj":"8477448"},{"id":"25943524-17671506-43313278","span":{"begin":189,"end":193},"obj":"17671506"},{"id":"25943524-10567534-43313279","span":{"begin":211,"end":215},"obj":"10567534"},{"id":"25943524-8477448-43313280","span":{"begin":511,"end":515},"obj":"8477448"},{"id":"25943524-10567534-43313281","span":{"begin":533,"end":537},"obj":"10567534"},{"id":"25943524-10567534-43313282","span":{"begin":671,"end":675},"obj":"10567534"}],"attributes":[{"subj":"25943524-8477448-43313277","pred":"source","obj":"2_test"},{"subj":"25943524-17671506-43313278","pred":"source","obj":"2_test"},{"subj":"25943524-10567534-43313279","pred":"source","obj":"2_test"},{"subj":"25943524-8477448-43313280","pred":"source","obj":"2_test"},{"subj":"25943524-10567534-43313281","pred":"source","obj":"2_test"},{"subj":"25943524-10567534-43313282","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ece3","default":true}]}]}}