PMC:4502371 / 27504-28482 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502371","sourcedb":"PMC","sourceid":"4502371","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502371","text":"Four suppressors (S11, S18, S19, S23) looked very similar to each other and shorter in body length than wild-type, exhibiting the Dpy phenotype. They failed to complement dpy-17(e164), indicating that they all carry mutations in dpy-17. We isolated dpy-17 mutations from two independently performed screens (S11 is from the first screen; S18, S19, and S23 are from the second screen), strongly suggesting that dpy-17 mutations suppress mua-3 lethality. S11 is close to sterile, probably due to other mutations; we excluded it from further study. To confirm dpy-17 suppression of mua-3 lethality, we treated mua-3 mutants with dpy-17 RNAi. dpy-17 RNAi rescued mua-3 lethality (Figure 6B), validating dpy-17 as a suppressor. The maximum rescue rate we saw was approximately 50% survival. Only approximately 50% of wild-type animals treated with dpy-17 RNAi showed a clear Dpy phenotype (data not shown), suggesting the low rescue percentage could be due to incomplete RNAi effect.","tracks":[]}