PMC:4502371 / 14925-16322 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502371","sourcedb":"PMC","sourceid":"4502371","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502371","text":"Figure 1 Characterization of a newly isolated mua-3 allele, uy19. (A) Temperature-dependent lethality of the newly isolated mua-3 mutant. The numbers of dead animals and total animals were counted to calculate percent survival. Animals were synchronized and plated as L1s and the percentage of survival was measured after they passed the L4 molt; 61 hr for 15°, 40 hr for 20°, and 30 hr for 25°. The numbers are average percent survival ± SEM. (B) A schematic drawing of the exons and introns of mua-3 gene and the location of the deletion. (C) A schematic drawing of the domain structure of the mua-3 gene (Bercher et al. 2001) and the location of the deletion. (D) Time course of death of the mua-3(uy19) mutants at 25°. Very few deaths were observed until 30 hr from L1. Then, within 3–4 hr, more than 90% were dead. The time window of death was somewhat wider than a worm’s molting period (approximately 2 hr) because the mutants are not perfectly synchronized, and thus the time to reach the molt varies among the animals. The numbers are average percent survival ± SEM. (E) A phylogenetic tree to show MUA-3 is a homolog of human fibrillins. Compared fibrillin homologs are C. elegans MUA-3, C. elegans MUP-4, C. elegans FBN-1, Drosophila melanogaster DP, Homo sapiens FBN1, FBN2, and FBN3. H. sapiens EYS sequence was included as an outgroup member to infer the root of this unrooted tree.","divisions":[{"label":"label","span":{"begin":0,"end":8}}],"tracks":[{"project":"2_test","denotations":[{"id":"25917920-11470828-43299632","span":{"begin":624,"end":628},"obj":"11470828"}],"attributes":[{"subj":"25917920-11470828-43299632","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#a9ec93","default":true}]}]}}