PMC:4502370 / 42996-43945 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502370","sourcedb":"PMC","sourceid":"4502370","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502370","text":"In a separate approach entailing a genomic library screen, we identified RPO26 as a dosage suppressor of the cold-sensitive phenotype of tgs1∆ cells. Because even a nominally single extra copy of the RPO26 gene on a CEN plasmid revived tgs1∆ growth at 18°, we surmise that RPO26 is an especially vulnerable target of the effect of the tgs1∆ mutation. This vulnerability is unlikely to reflect a fastidious gene-specific requirement for TMG caps or other splicing factors in removing the RPO26 intron, insofar as the RPO26 5′-splice site, branchpoint, and 3′-splice site adhere perfectly to the yeast consensus sequences and the intron is situated close to the 5′ end of the RPO26 ORF, as is the case for most yeast genes. Rather, it is the fact that even small changes in RPO26 expression can result in an overt growth defect in the cold (Nouraini et al. 1996b) that allowed us to recover a singularly sensitive target gene in the suppressor screen.","tracks":[]}