PMC:4502370 / 39572-40646 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4502370","sourcedb":"PMC","sourceid":"4502370","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502370","text":"The present study provides new genetic insights to the impact of the lack of TMG caps in budding yeast. Prior screening for synthetic lethal and sick tgs1∆ interactions had drawn attention specifically to the U1 snRNP as a focal point for TMG cap function in vivo (Hausmann et al. 2008). This idea was fortified by the findings that the only overt change in the composition of yeast spliceosomal snRNPs in tgs1∆ cells was the gain of CBC as a stoichiometric component of the U1 snRNP, by virtue of its binding to the residual m7G cap on the U1 snRNA (Schwer et al. 2011). In TGS1 cells, CBC is loosely associated with the U1 snRNP at low sub-stoichiometric levels compared to the intrinsic U1 snRNP subunits (Schwer et al. 2011). It is thought that CBC interacts with one or more of the U1 snRNP proteins to facilitate bridging interactions between CBC bound to the pre-mRNA m7G cap and the U1 snRNP at the 5′ splice site (Lewis et al. 1996; Görnemann et al. 2005). Hypomorphic mutations in Cbc2 that weaken cap binding suppress the tgs1∆ cs growth defect (Qiu et al. 2012).","tracks":[{"project":"2_test","denotations":[{"id":"25911228-18775984-43354281","span":{"begin":281,"end":285},"obj":"18775984"},{"id":"25911228-21558325-43354282","span":{"begin":565,"end":569},"obj":"21558325"},{"id":"25911228-21558325-43354283","span":{"begin":723,"end":727},"obj":"21558325"},{"id":"25911228-8682298-43354284","span":{"begin":936,"end":940},"obj":"8682298"},{"id":"25911228-15989964-43354285","span":{"begin":959,"end":963},"obj":"15989964"},{"id":"25911228-23002122-43354286","span":{"begin":1068,"end":1072},"obj":"23002122"}],"attributes":[{"subj":"25911228-18775984-43354281","pred":"source","obj":"2_test"},{"subj":"25911228-21558325-43354282","pred":"source","obj":"2_test"},{"subj":"25911228-21558325-43354283","pred":"source","obj":"2_test"},{"subj":"25911228-8682298-43354284","pred":"source","obj":"2_test"},{"subj":"25911228-15989964-43354285","pred":"source","obj":"2_test"},{"subj":"25911228-23002122-43354286","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ecdc","default":true}]}]}}