PMC:4502370 / 33840-34724 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502370","sourcedb":"PMC","sourceid":"4502370","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502370","text":"We then tested the effects of deleting 5 amino acids or 16 amino acids from the C-terminus of the biologically active Rpo26-(70-155) polypeptide. Whereas RPO26-(70-150) was able to support growth of tgs1∆ cells at 18°, the RPO26-(70-140) allele was not (Figure 5). RPO26-(70-140) failed to complement rpo26∆ (not shown). By contrast, RPO26-(70-150) did complement rpo26∆, albeit with a conditional phenotype whereby RPO26-(70-150) cells grew well at 18° and 25°, formed small colonies at 30°, and failed to grow at 37° (Figure 6B). Thus, the decapeptide segment 141GSFEDWSVEE150 is necessary for Rpo21 function at warmer temperatures. Because a previous study had shown that a nonsense mutant allele encoding Rpo26-(1-145) was unable to complement rpo26∆ (Nouraini et al. 1996a), we can surmise that the pentapeptide 146WSVEE150 contains features essential for Rpo26 activity in vivo.","tracks":[]}