PMC:4502370 / 2920-3873 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502370","sourcedb":"PMC","sourceid":"4502370","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502370","text":"S. cerevisiae can grow in the absence of Tgs1 because the effects of ablating the TMG cap of the spliceosomal U snRNAs are genetically buffered, either by spliceosome assembly factors that are themselves inessential for vegetative growth (Hausmann et al. 2008; Wilmes et al. 2008; Chang et al. 2010) or by otherwise dispensable domains of the essential branchpoint binding protein Msl5 (Chang et al. 2012). Nonetheless, there are two situations in which the lack of TMG caps per se elicits a profound phenotype. First, S. cerevisiae tgs1∆ diploids are unable to properly execute meiosis and sporulation because they are defective in splicing certain meiotic pre-mRNAs (Qiu et al. 2011). Second, although S. cerevisiae haploid tgs1∆ cells grow normally at 30°–37°, they are unable to grow at 18°–20° (Mouaikel et al. 2002; Hausmann et al. 2008), signifying that one or more essential cellular transactions becomes reliant on TMG caps at low temperatures.","tracks":[]}