PMC:4502369 / 32475-33619 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502369","sourcedb":"PMC","sourceid":"4502369","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502369","text":"Differential expressions identified in the RNAseq data were validated by RT-PCR of selected genes in the testis and oviduct tissue samples. For the validation, we selected DE genes associated with reproduction that contained nonsynonymous SNPs, because these genes represent potential candidate genes for an effect in reproduction traits. We analyzed the expression differences between the testis and oviduct samples for four genes (FRAS1, TCF4, ADAT1, and SPAG6) by qPCR and for two additional genes (PIWIL2 and DNAH8) by RT-PCR and an agarose gel. All genes showed a similar expression pattern in the RT-PCR analysis, as detected by RNAseq (Figure 5). Testis-specific genes ADAT1, SPAG6, PIWIL2, and DNAH8 exhibited none or extremely low expression in the oviduct. The genes with higher expression in the oviduct compared to the testis in the RNAseq data appeared to be present in the testis samples, but at a much lower level (Figure 5B). Furthermore, the polymorphisms within genes FRAS1, ADAT1, SPAG6, DNAH8, and PGR were confirmed by Sanger sequencing. Thus, these polymorphisms represent potential candidates for gene-assisted selection.","tracks":[]}