PMC:4502369 / 18033-19961 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502369","sourcedb":"PMC","sourceid":"4502369","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502369","text":"Differentially expressed genes in the testis and oviduct\nThe analysis of gene expression differences between the testis and oviduct highlighted 1234 upregulated genes in the testis and 1501 in the oviduct. Significantly enriched GO terms (P \u003c 10−5) in the testis included acrosome reaction, sperm–egg recognition, sperm motility, spermatid development, cell-cycle process, and spermatogenesis (Figure 1A). In the oviduct, more diverse processes were significant, including signaling, regulation, and developmental processes (Figure 1B). The top 30 DE genes based on fold change (FC) are listed in Table S3. Genes with a high FC between the testis and oviduct resulted in enrichment in sperm motility (SMCP, TNP1), spermatogenesis (INSL3, PRM1, ADAM29, SPEM), and histone exchange and nucleosome disassembly (HIST1H2BA, TNP1) in the testis and in muscle contraction (DES, TPM2, TNNC1, ACTG2), extracellular negative regulation of signal transduction (AGTR2, LTBP1), and mesenchymal-epithelial cell signaling (TNC, HOXA5) in the oviduct. Several pseudogenes (LOC100510878, LOC100516119, LOC100519930, and LOC100626054) and ncRNA (LOC100523888, Spty2d1-AS1) were also specifically expressed among the top 30 expressed genes in the testis. In total, 47 genes with a known role in reproduction were upregulated in the oviduct and 52 genes were upregulated in the testis (Table S4). These reproduction-related genes appear to be sex-specific, underlining possible candidate genes for reproductive performance in the pig. In total, 440 genes with a biological role in reproduction were identified in the dataset based on human gene names. Gene names were identified and recognized by Panther tool for 10,320 expressed genes.\nFigure 1 Enriched GO terms among upregulated genes (P \u003c 10−5). (A) Enriched biological processes among upregulated genes in the testis. (B) Enriched biological processes among upregulated genes in the oviduct.","divisions":[{"label":"title","span":{"begin":0,"end":56}},{"label":"p","span":{"begin":57,"end":1717}},{"label":"label","span":{"begin":1718,"end":1726}}],"tracks":[]}