PMC:4419340 / 32655-33194 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4419340","sourcedb":"PMC","sourceid":"4419340","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4419340","text":"Fibroblast activation.\nSV40-transformed fibroblasts were plated in 48-well plates at a density of 50,000 cells/well in 0.5 ml DMEM/10% FBS per well. The cells were incubated for 24 h and were then left unstimulated or were stimulated for 24 h with various amounts of IL-17 cytokines, recombinant human IL-17A, IL-17F, IL-17A/F, and IL-1β (from R\u0026D Systems). 24 h later, supernatants were collected and IL-6 (Sanquin or R\u0026D Systems) and GRO-α (R\u0026D Systems) levels were assessed with ELISA kits, according to the manufacturer’s instructions.","divisions":[{"label":"Title","span":{"begin":0,"end":22}}],"tracks":[]}