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PMC:441373 / 48337-49764 JSONTXT

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Id Subject Object Predicate Lexical cue
T12128 0-3 DT denotes The
T12129 10-14 NN denotes cDNA
T12130 4-9 NN denotes TACC2
T12131 19-25 VBN denotes cloned
T12132 15-18 VBD denotes was
T12133 26-30 IN denotes into
T12134 31-34 NN denotes GST
T12135 49-56 NN denotes pGEX5X2
T12136 35-41 NN denotes fusion
T12137 42-48 NN denotes vector
T12138 57-58 -LRB- denotes (
T12139 67-78 NNP denotes Biosciences
T12140 58-66 NNP denotes Amersham
T12141 78-80 , denotes ,
T12142 80-90 NNP denotes Piscataway
T12143 90-92 , denotes ,
T12144 92-94 NNP denotes NJ
T12145 94-96 , denotes ,
T12146 96-99 NNP denotes USA
T12147 99-100 -RRB- denotes )
T12148 100-101 . denotes .
T12149 101-214 sentence denotes GST and GST-TACC2 proteins were expressed in E. coli BL21(DE3) plys "S" with 1 mM IPTG at 37°C shaker for 2 hrs.
T12150 102-105 NN denotes GST
T12151 120-128 NN denotes proteins
T12152 106-109 CC denotes and
T12153 110-113 NN denotes GST
T12154 114-119 NN denotes TACC2
T12155 113-114 HYPH denotes -
T12156 134-143 VBN denotes expressed
T12157 129-133 VBD denotes were
T12158 144-146 IN denotes in
T12159 147-149 NNP denotes E.
T12160 150-154 NNP denotes coli
T12161 171-172 NN denotes S
T12162 155-159 NN denotes BL21
T12163 159-160 -LRB- denotes (
T12164 160-163 NN denotes DE3
T12165 163-164 -RRB- denotes )
T12166 165-169 NNS denotes plys
T12167 170-171 `` denotes "
T12168 172-173 '' denotes "
T12169 174-178 IN denotes with
T12170 179-180 CD denotes 1
T12171 181-183 NN denotes mM
T12172 184-188 NN denotes IPTG
T12173 189-191 IN denotes at
T12174 192-194 CD denotes 37
T12175 194-196 NN denotes °C
T12176 197-203 NN denotes shaker
T12177 204-207 IN denotes for
T12178 208-209 CD denotes 2
T12179 210-213 NNS denotes hrs
T12180 213-214 . denotes .
T12181 214-364 sentence denotes Cells (50 ml) were harvested and resuspended in 5 ml of 20 mM Tris-HCl pH.8.0, 200 mM NaCl, 1 mM EDTA pH8.0, Protease inhibitor set III (Calbiochem).
T12182 215-220 NNS denotes Cells
T12183 234-243 VBN denotes harvested
T12184 221-222 -LRB- denotes (
T12185 225-227 NN denotes ml
T12186 222-224 CD denotes 50
T12187 227-228 -RRB- denotes )
T12188 229-233 VBD denotes were
T12189 244-247 CC denotes and
T12190 248-259 VBN denotes resuspended
T12191 260-262 IN denotes in
T12192 263-264 CD denotes 5
T12193 265-267 NN denotes ml
T12194 268-270 IN denotes of
T12195 271-273 CD denotes 20
T12196 274-276 NN denotes mM
T12197 282-285 NN denotes HCl
T12198 277-281 NN denotes Tris
T12199 281-282 HYPH denotes -
T12200 286-292 NN denotes pH.8.0
T12201 292-294 , denotes ,
T12202 294-297 CD denotes 200
T12203 298-300 NN denotes mM
T12204 301-305 NN denotes NaCl
T12205 305-307 , denotes ,
T12206 307-308 CD denotes 1
T12207 309-311 NN denotes mM
T12208 312-316 NN denotes EDTA
T12209 317-322 NN denotes pH8.0
T12210 322-324 , denotes ,
T12211 324-332 NN denotes Protease
T12212 333-342 NN denotes inhibitor
T12213 343-346 NN denotes set
T12214 347-350 CD denotes III
T12215 351-352 -LRB- denotes (
T12216 352-362 NNP denotes Calbiochem
T12217 362-363 -RRB- denotes )
T12218 363-364 . denotes .
T12219 364-467 sentence denotes The cells were lysed by sonication and lysate cleared by centrifugation at 7500 rpm at 4°C for 15 min.
T12220 365-368 DT denotes The
T12221 369-374 NNS denotes cells
T12222 380-385 VBN denotes lysed
T12223 375-379 VBD denotes were
T12224 386-388 IN denotes by
T12225 389-399 NN denotes sonication
T12226 400-403 CC denotes and
T12227 404-410 NN denotes lysate
T12228 411-418 VBN denotes cleared
T12229 419-421 IN denotes by
T12230 422-436 NN denotes centrifugation
T12231 437-439 IN denotes at
T12232 440-444 CD denotes 7500
T12233 445-448 NN denotes rpm
T12234 449-451 IN denotes at
T12235 452-453 CD denotes 4
T12236 453-455 NN denotes °C
T12237 456-459 IN denotes for
T12238 460-462 CD denotes 15
T12239 463-466 NN denotes min
T12240 466-467 . denotes .
T12241 467-598 sentence denotes The cleared lysate was immobilized on glutathione sepharose beads in 3 ml of 20 mM Tris-HCl pH.8.0, 200 mM NaCl, 1 mM EDTA pH8.0).
T12242 468-471 DT denotes The
T12243 480-486 NN denotes lysate
T12244 472-479 JJ denotes cleared
T12245 491-502 VBN denotes immobilized
T12246 487-490 VBD denotes was
T12247 503-505 IN denotes on
T12248 506-517 NN denotes glutathione
T12249 518-527 NN denotes sepharose
T12250 528-533 NNS denotes beads
T12251 534-536 IN denotes in
T12252 537-538 CD denotes 3
T12253 539-541 NN denotes ml
T12254 542-544 IN denotes of
T12255 545-547 CD denotes 20
T12256 548-550 NN denotes mM
T12257 556-559 NN denotes HCl
T12258 551-555 NN denotes Tris
T12259 555-556 HYPH denotes -
T12260 560-566 NN denotes pH.8.0
T12261 566-568 , denotes ,
T12262 568-571 CD denotes 200
T12263 572-574 NN denotes mM
T12264 575-579 NN denotes NaCl
T12265 579-581 , denotes ,
T12266 581-582 CD denotes 1
T12267 583-585 NN denotes mM
T12268 586-590 NN denotes EDTA
T12269 591-596 NN denotes pH8.0
T12270 596-597 -RRB- denotes )
T12271 597-598 . denotes .
T12272 598-839 sentence denotes RXRβ3 cDNA was cloned into pET 28C(+) (Invitrogen, Carlsbad, CA, USA) and protein synthesized by TNT quick coupled transcription/translation system kit (Promega) and radiolabeled with 35S methionine according to manufacturer's instructions.
T12273 599-604 NN denotes RXRβ3
T12274 605-609 NN denotes cDNA
T12275 614-620 VBN denotes cloned
T12276 610-613 VBD denotes was
T12277 621-625 IN denotes into
T12278 626-629 NN denotes pET
T12279 630-633 NN denotes 28C
T12280 633-634 -LRB- denotes (
T12281 634-635 SYM denotes +
T12282 635-636 -RRB- denotes )
T12283 637-638 -LRB- denotes (
T12284 638-648 NNP denotes Invitrogen
T12285 648-650 , denotes ,
T12286 650-658 NNP denotes Carlsbad
T12287 658-660 , denotes ,
T12288 660-662 NNP denotes CA
T12289 662-664 , denotes ,
T12290 664-667 NNP denotes USA
T12291 667-668 -RRB- denotes )
T12292 669-672 CC denotes and
T12293 673-680 NN denotes protein
T12294 681-692 VBN denotes synthesized
T12295 693-695 IN denotes by
T12296 696-699 NN denotes TNT
T12297 747-750 NN denotes kit
T12298 700-705 RB denotes quick
T12299 706-713 VBN denotes coupled
T12300 714-727 NN denotes transcription
T12301 728-739 NN denotes translation
T12302 727-728 HYPH denotes /
T12303 740-746 NN denotes system
T12304 751-752 -LRB- denotes (
T12305 752-759 NNP denotes Promega
T12306 759-760 -RRB- denotes )
T12307 761-764 CC denotes and
T12308 765-777 VBN denotes radiolabeled
T12309 778-782 IN denotes with
T12310 783-786 NN denotes 35S
T12311 787-797 NN denotes methionine
T12312 798-807 VBG denotes according
T12313 808-810 IN denotes to
T12314 811-823 NN denotes manufacturer
T12315 826-838 NNS denotes instructions
T12316 823-825 POS denotes 's
T12317 838-839 . denotes .
T12318 839-1013 sentence denotes 100 μl of in vitro translated RXRβ3 protein in 1 ml of 20 mM Tris-HCl pH.8.0, 200 mM NaCl, 1 mM EDTA pH8.0 was incubated at 4°C with immobilized GST-TACC2 or GST for 90 min.
T12319 840-843 CD denotes 100
T12320 844-846 NN denotes μl
T12321 951-960 VBN denotes incubated
T12322 847-849 IN denotes of
T12323 850-852 FW denotes in
T12324 853-858 FW denotes vitro
T12325 859-869 VBN denotes translated
T12326 876-883 NN denotes protein
T12327 870-875 NN denotes RXRβ3
T12328 884-886 IN denotes in
T12329 887-888 CD denotes 1
T12330 889-891 NN denotes ml
T12331 892-894 IN denotes of
T12332 895-897 CD denotes 20
T12333 898-900 NN denotes mM
T12334 906-909 NN denotes HCl
T12335 901-905 NN denotes Tris
T12336 905-906 HYPH denotes -
T12337 910-916 NN denotes pH.8.0
T12338 916-918 , denotes ,
T12339 918-921 CD denotes 200
T12340 922-924 NN denotes mM
T12341 925-929 NN denotes NaCl
T12342 929-931 , denotes ,
T12343 931-932 CD denotes 1
T12344 933-935 NN denotes mM
T12345 936-940 NN denotes EDTA
T12346 941-946 NN denotes pH8.0
T12347 947-950 VBD denotes was
T12348 961-963 IN denotes at
T12349 964-965 CD denotes 4
T12350 965-967 NN denotes °C
T12351 968-972 IN denotes with
T12352 973-984 VBN denotes immobilized
T12353 989-994 NN denotes TACC2
T12354 985-988 NN denotes GST
T12355 988-989 HYPH denotes -
T12356 995-997 CC denotes or
T12357 998-1001 NN denotes GST
T12358 1002-1005 IN denotes for
T12359 1006-1008 CD denotes 90
T12360 1009-1012 NN denotes min
T12361 1012-1013 . denotes .
T12362 1013-1120 sentence denotes Unbound RXRβ3 was removed by washing three times with 20 mM Tris-HCl pH.8.0, 200 mM NaCl, 1 mM EDTA pH8.0.
T12363 1014-1021 JJ denotes Unbound
T12364 1022-1027 NN denotes RXRβ3
T12365 1032-1039 VBN denotes removed
T12366 1028-1031 VBD denotes was
T12367 1040-1042 IN denotes by
T12368 1043-1050 VBG denotes washing
T12369 1051-1056 CD denotes three
T12370 1057-1062 NNS denotes times
T12371 1063-1067 IN denotes with
T12372 1068-1070 CD denotes 20
T12373 1071-1073 NN denotes mM
T12374 1079-1082 NN denotes HCl
T12375 1074-1078 NN denotes Tris
T12376 1078-1079 HYPH denotes -
T12377 1083-1089 NN denotes pH.8.0
T12378 1089-1091 , denotes ,
T12379 1091-1094 CD denotes 200
T12380 1095-1097 NN denotes mM
T12381 1098-1102 NN denotes NaCl
T12382 1102-1104 , denotes ,
T12383 1104-1105 CD denotes 1
T12384 1106-1108 NN denotes mM
T12385 1109-1113 NN denotes EDTA
T12386 1114-1119 NN denotes pH8.0
T12387 1119-1120 . denotes .
T12388 1120-1264 sentence denotes Bound proteins were eluted from the beads at room temperature for 10 min in elution buffer (100 mM Tris HCl, pH8.0, 20 mM reduced glutathione).
T12389 1121-1126 NN denotes Bound
T12390 1127-1135 NN denotes proteins
T12391 1141-1147 VBN denotes eluted
T12392 1136-1140 VBD denotes were
T12393 1148-1152 IN denotes from
T12394 1153-1156 DT denotes the
T12395 1157-1162 NNS denotes beads
T12396 1163-1165 IN denotes at
T12397 1166-1170 NN denotes room
T12398 1171-1182 NN denotes temperature
T12399 1183-1186 IN denotes for
T12400 1187-1189 CD denotes 10
T12401 1190-1193 NN denotes min
T12402 1194-1196 IN denotes in
T12403 1197-1204 NN denotes elution
T12404 1205-1211 NN denotes buffer
T12405 1212-1213 -LRB- denotes (
T12406 1225-1228 NN denotes HCl
T12407 1213-1216 CD denotes 100
T12408 1217-1219 NN denotes mM
T12409 1220-1224 NN denotes Tris
T12410 1228-1230 , denotes ,
T12411 1230-1235 NN denotes pH8.0
T12412 1235-1237 , denotes ,
T12413 1237-1239 CD denotes 20
T12414 1240-1242 NN denotes mM
T12415 1251-1262 NN denotes glutathione
T12416 1243-1250 VBN denotes reduced
T12417 1262-1263 -RRB- denotes )
T12418 1263-1264 . denotes .
T12419 1264-1323 sentence denotes The proteins were analyzed on 12% SDS polyacrylamide gels.
T12420 1265-1268 DT denotes The
T12421 1269-1277 NN denotes proteins
T12422 1283-1291 VBN denotes analyzed
T12423 1278-1282 VBD denotes were
T12424 1292-1294 IN denotes on
T12425 1295-1297 CD denotes 12
T12426 1297-1298 NN denotes %
T12427 1318-1322 NNS denotes gels
T12428 1299-1302 NN denotes SDS
T12429 1303-1317 NN denotes polyacrylamide
T12430 1322-1323 . denotes .
T12431 1323-1393 sentence denotes Coomassie blue staining verified equal loading of GST fusion protein.
T12432 1324-1333 NNP denotes Coomassie
T12433 1339-1347 NN denotes staining
T12434 1334-1338 JJ denotes blue
T12435 1348-1356 VBD denotes verified
T12436 1357-1362 JJ denotes equal
T12437 1363-1370 NN denotes loading
T12438 1371-1373 IN denotes of
T12439 1374-1377 NN denotes GST
T12440 1385-1392 NN denotes protein
T12441 1378-1384 NN denotes fusion
T12442 1392-1393 . denotes .
T12443 1393-1427 sentence denotes Dried gels were autoradiographed.
T12444 1394-1399 VBN denotes Dried
T12445 1400-1404 NNS denotes gels
T12446 1410-1426 VBN denotes autoradiographed
T12447 1405-1409 VBD denotes were
T12448 1426-1427 . denotes .
R7780 T12128 T12129 det The,cDNA
R7781 T12129 T12131 nsubjpass cDNA,cloned
R7782 T12130 T12129 compound TACC2,cDNA
R7783 T12132 T12131 auxpass was,cloned
R7784 T12133 T12131 prep into,cloned
R7785 T12134 T12135 compound GST,pGEX5X2
R7786 T12135 T12133 pobj pGEX5X2,into
R7787 T12136 T12135 compound fusion,pGEX5X2
R7788 T12137 T12135 compound vector,pGEX5X2
R7789 T12138 T12139 punct (,Biosciences
R7790 T12139 T12131 parataxis Biosciences,cloned
R7791 T12140 T12139 compound Amersham,Biosciences
R7792 T12141 T12139 punct ", ",Biosciences
R7793 T12142 T12139 npadvmod Piscataway,Biosciences
R7794 T12143 T12139 punct ", ",Biosciences
R7795 T12144 T12139 npadvmod NJ,Biosciences
R7796 T12145 T12139 punct ", ",Biosciences
R7797 T12146 T12139 npadvmod USA,Biosciences
R7798 T12147 T12139 punct ),Biosciences
R7799 T12148 T12131 punct .,cloned
R7800 T12150 T12151 nmod GST,proteins
R7801 T12151 T12156 nsubjpass proteins,expressed
R7802 T12152 T12150 cc and,GST
R7803 T12153 T12154 compound GST,TACC2
R7804 T12154 T12150 conj TACC2,GST
R7805 T12155 T12154 punct -,TACC2
R7806 T12157 T12156 auxpass were,expressed
R7807 T12158 T12156 prep in,expressed
R7808 T12159 T12160 nmod E.,coli
R7809 T12160 T12161 nmod coli,S
R7810 T12161 T12158 pobj S,in
R7811 T12162 T12161 nmod BL21,S
R7812 T12163 T12164 punct (,DE3
R7813 T12164 T12161 parataxis DE3,S
R7814 T12165 T12164 punct ),DE3
R7815 T12166 T12161 nmod plys,S
R7816 T12167 T12161 punct """",S
R7817 T12168 T12161 punct """",S
R7818 T12169 T12156 prep with,expressed
R7819 T12170 T12171 nummod 1,mM
R7820 T12171 T12172 compound mM,IPTG
R7821 T12172 T12169 pobj IPTG,with
R7822 T12173 T12172 prep at,IPTG
R7823 T12174 T12175 nummod 37,°C
R7824 T12175 T12176 compound °C,shaker
R7825 T12176 T12173 pobj shaker,at
R7826 T12177 T12172 prep for,IPTG
R7827 T12178 T12179 nummod 2,hrs
R7828 T12179 T12177 pobj hrs,for
R7829 T12180 T12156 punct .,expressed
R7830 T12182 T12183 nsubjpass Cells,harvested
R7831 T12184 T12185 punct (,ml
R7832 T12185 T12182 parataxis ml,Cells
R7833 T12186 T12185 nummod 50,ml
R7834 T12187 T12185 punct ),ml
R7835 T12188 T12183 auxpass were,harvested
R7836 T12189 T12183 cc and,harvested
R7837 T12190 T12183 conj resuspended,harvested
R7838 T12191 T12190 prep in,resuspended
R7839 T12192 T12193 nummod 5,ml
R7840 T12193 T12191 pobj ml,in
R7841 T12194 T12193 prep of,ml
R7842 T12195 T12196 nummod 20,mM
R7843 T12196 T12197 compound mM,HCl
R7844 T12197 T12194 pobj HCl,of
R7845 T12198 T12197 compound Tris,HCl
R7846 T12199 T12197 punct -,HCl
R7847 T12200 T12197 npadvmod pH.8.0,HCl
R7848 T12201 T12197 punct ", ",HCl
R7849 T12202 T12203 nummod 200,mM
R7850 T12203 T12204 compound mM,NaCl
R7851 T12204 T12197 appos NaCl,HCl
R7852 T12205 T12197 punct ", ",HCl
R7853 T12206 T12207 nummod 1,mM
R7854 T12207 T12208 compound mM,EDTA
R7855 T12208 T12197 appos EDTA,HCl
R7856 T12209 T12208 npadvmod pH8.0,EDTA
R7857 T12210 T12197 punct ", ",HCl
R7858 T12211 T12212 compound Protease,inhibitor
R7859 T12212 T12213 compound inhibitor,set
R7860 T12213 T12197 appos set,HCl
R7861 T12214 T12213 nummod III,set
R7862 T12215 T12216 punct (,Calbiochem
R7863 T12216 T12213 parataxis Calbiochem,set
R7864 T12217 T12216 punct ),Calbiochem
R7865 T12218 T12183 punct .,harvested
R7866 T12220 T12221 det The,cells
R7867 T12221 T12222 nsubjpass cells,lysed
R7868 T12223 T12222 auxpass were,lysed
R7869 T12224 T12222 prep by,lysed
R7870 T12225 T12224 pobj sonication,by
R7871 T12226 T12222 cc and,lysed
R7872 T12227 T12228 nsubj lysate,cleared
R7873 T12228 T12222 conj cleared,lysed
R7874 T12229 T12228 prep by,cleared
R7875 T12230 T12229 pobj centrifugation,by
R7876 T12231 T12230 prep at,centrifugation
R7877 T12232 T12233 nummod 7500,rpm
R7878 T12233 T12231 pobj rpm,at
R7879 T12234 T12230 prep at,centrifugation
R7880 T12235 T12236 nummod 4,°C
R7881 T12236 T12234 pobj °C,at
R7882 T12237 T12230 prep for,centrifugation
R7883 T12238 T12239 nummod 15,min
R7884 T12239 T12237 pobj min,for
R7885 T12240 T12228 punct .,cleared
R7886 T12242 T12243 det The,lysate
R7887 T12243 T12245 nsubjpass lysate,immobilized
R7888 T12244 T12243 amod cleared,lysate
R7889 T12246 T12245 auxpass was,immobilized
R7890 T12247 T12245 prep on,immobilized
R7891 T12248 T12249 compound glutathione,sepharose
R7892 T12249 T12250 compound sepharose,beads
R7893 T12250 T12247 pobj beads,on
R7894 T12251 T12245 prep in,immobilized
R7895 T12252 T12253 nummod 3,ml
R7896 T12253 T12251 pobj ml,in
R7897 T12254 T12253 prep of,ml
R7898 T12255 T12256 nummod 20,mM
R7899 T12256 T12257 compound mM,HCl
R7900 T12257 T12254 pobj HCl,of
R7901 T12258 T12257 compound Tris,HCl
R7902 T12259 T12257 punct -,HCl
R7903 T12260 T12257 npadvmod pH.8.0,HCl
R7904 T12261 T12257 punct ", ",HCl
R7905 T12262 T12263 nummod 200,mM
R7906 T12263 T12264 compound mM,NaCl
R7907 T12264 T12257 appos NaCl,HCl
R7908 T12265 T12257 punct ", ",HCl
R7909 T12266 T12267 nummod 1,mM
R7910 T12267 T12268 compound mM,EDTA
R7911 T12268 T12257 appos EDTA,HCl
R7912 T12269 T12268 npadvmod pH8.0,EDTA
R7913 T12270 T12245 punct ),immobilized
R7914 T12271 T12245 punct .,immobilized
R7915 T12273 T12274 compound RXRβ3,cDNA
R7916 T12274 T12275 nsubjpass cDNA,cloned
R7917 T12276 T12275 auxpass was,cloned
R7918 T12277 T12275 prep into,cloned
R7919 T12278 T12279 compound pET,28C
R7920 T12279 T12277 pobj 28C,into
R7921 T12280 T12279 punct (,28C
R7922 T12281 T12279 punct +,28C
R7923 T12282 T12279 punct ),28C
R7924 T12283 T12284 punct (,Invitrogen
R7925 T12284 T12279 parataxis Invitrogen,28C
R7926 T12285 T12284 punct ", ",Invitrogen
R7927 T12286 T12284 npadvmod Carlsbad,Invitrogen
R7928 T12287 T12284 punct ", ",Invitrogen
R7929 T12288 T12284 npadvmod CA,Invitrogen
R7930 T12289 T12284 punct ", ",Invitrogen
R7931 T12290 T12284 npadvmod USA,Invitrogen
R7932 T12291 T12284 punct ),Invitrogen
R7933 T12292 T12275 cc and,cloned
R7934 T12293 T12294 nsubj protein,synthesized
R7935 T12294 T12275 conj synthesized,cloned
R7936 T12295 T12294 prep by,synthesized
R7937 T12296 T12297 nmod TNT,kit
R7938 T12297 T12295 pobj kit,by
R7939 T12298 T12299 advmod quick,coupled
R7940 T12299 T12297 amod coupled,kit
R7941 T12300 T12301 compound transcription,translation
R7942 T12301 T12297 compound translation,kit
R7943 T12302 T12301 punct /,translation
R7944 T12303 T12297 compound system,kit
R7945 T12304 T12305 punct (,Promega
R7946 T12305 T12297 parataxis Promega,kit
R7947 T12306 T12305 punct ),Promega
R7948 T12307 T12294 cc and,synthesized
R7949 T12308 T12294 conj radiolabeled,synthesized
R7950 T12309 T12308 prep with,radiolabeled
R7951 T12310 T12311 compound 35S,methionine
R7952 T12311 T12309 pobj methionine,with
R7953 T12312 T12308 prep according,radiolabeled
R7954 T12313 T12312 prep to,according
R7955 T12314 T12315 poss manufacturer,instructions
R7956 T12315 T12313 pobj instructions,to
R7957 T12316 T12314 case 's,manufacturer
R7958 T12317 T12294 punct .,synthesized
R7959 T12319 T12320 nummod 100,μl
R7960 T12320 T12321 nsubjpass μl,incubated
R7961 T12322 T12320 prep of,μl
R7962 T12323 T12324 advmod in,vitro
R7963 T12324 T12325 advmod vitro,translated
R7964 T12325 T12326 amod translated,protein
R7965 T12326 T12322 pobj protein,of
R7966 T12327 T12326 compound RXRβ3,protein
R7967 T12328 T12320 prep in,μl
R7968 T12329 T12330 nummod 1,ml
R7969 T12330 T12328 pobj ml,in
R7970 T12331 T12330 prep of,ml
R7971 T12332 T12333 nummod 20,mM
R7972 T12333 T12334 compound mM,HCl
R7973 T12334 T12331 pobj HCl,of
R7974 T12335 T12334 compound Tris,HCl
R7975 T12336 T12334 punct -,HCl
R7976 T12337 T12334 npadvmod pH.8.0,HCl
R7977 T12338 T12334 punct ", ",HCl
R7978 T12339 T12340 nummod 200,mM
R7979 T12340 T12341 compound mM,NaCl
R7980 T12341 T12334 appos NaCl,HCl
R7981 T12342 T12334 punct ", ",HCl
R7982 T12343 T12344 nummod 1,mM
R7983 T12344 T12345 compound mM,EDTA
R7984 T12345 T12334 appos EDTA,HCl
R7985 T12346 T12345 npadvmod pH8.0,EDTA
R7986 T12347 T12321 auxpass was,incubated
R7987 T12348 T12321 prep at,incubated
R7988 T12349 T12350 nummod 4,°C
R7989 T12350 T12348 pobj °C,at
R7990 T12351 T12321 prep with,incubated
R7991 T12352 T12353 amod immobilized,TACC2
R7992 T12353 T12351 pobj TACC2,with
R7993 T12354 T12353 compound GST,TACC2
R7994 T12355 T12353 punct -,TACC2
R7995 T12356 T12353 cc or,TACC2
R7996 T12357 T12353 conj GST,TACC2
R7997 T12358 T12321 prep for,incubated
R7998 T12359 T12360 nummod 90,min
R7999 T12360 T12358 pobj min,for
R8000 T12361 T12321 punct .,incubated
R8001 T12363 T12364 amod Unbound,RXRβ3
R8002 T12364 T12365 nsubjpass RXRβ3,removed
R8003 T12366 T12365 auxpass was,removed
R8004 T12367 T12365 prep by,removed
R8005 T12368 T12367 pcomp washing,by
R8006 T12369 T12370 nummod three,times
R8007 T12370 T12368 npadvmod times,washing
R8008 T12371 T12368 prep with,washing
R8009 T12372 T12373 nummod 20,mM
R8010 T12373 T12374 compound mM,HCl
R8011 T12374 T12371 pobj HCl,with
R8012 T12375 T12374 compound Tris,HCl
R8013 T12376 T12374 punct -,HCl
R8014 T12377 T12374 npadvmod pH.8.0,HCl
R8015 T12378 T12374 punct ", ",HCl
R8016 T12379 T12380 nummod 200,mM
R8017 T12380 T12381 compound mM,NaCl
R8018 T12381 T12374 appos NaCl,HCl
R8019 T12382 T12374 punct ", ",HCl
R8020 T12383 T12384 nummod 1,mM
R8021 T12384 T12385 compound mM,EDTA
R8022 T12385 T12374 appos EDTA,HCl
R8023 T12386 T12385 npadvmod pH8.0,EDTA
R8024 T12387 T12365 punct .,removed
R8025 T12389 T12390 compound Bound,proteins
R8026 T12390 T12391 nsubjpass proteins,eluted
R8027 T12392 T12391 auxpass were,eluted
R8028 T12393 T12391 prep from,eluted
R8029 T12394 T12395 det the,beads
R8030 T12395 T12393 pobj beads,from
R8031 T12396 T12391 prep at,eluted
R8032 T12397 T12398 compound room,temperature
R8033 T12398 T12396 pobj temperature,at
R8034 T12399 T12391 prep for,eluted
R8035 T12400 T12401 nummod 10,min
R8036 T12401 T12399 pobj min,for
R8037 T12402 T12391 prep in,eluted
R8038 T12403 T12404 compound elution,buffer
R8039 T12404 T12402 pobj buffer,in
R8040 T12405 T12406 punct (,HCl
R8041 T12406 T12404 parataxis HCl,buffer
R8042 T12407 T12408 nummod 100,mM
R8043 T12408 T12406 compound mM,HCl
R8044 T12409 T12406 compound Tris,HCl
R8045 T12410 T12406 punct ", ",HCl
R8046 T12411 T12406 npadvmod pH8.0,HCl
R8047 T12412 T12406 punct ", ",HCl
R8048 T12413 T12414 nummod 20,mM
R8049 T12414 T12415 nmod mM,glutathione
R8050 T12415 T12406 appos glutathione,HCl
R8051 T12416 T12415 amod reduced,glutathione
R8052 T12417 T12406 punct ),HCl
R8053 T12418 T12391 punct .,eluted
R8054 T12420 T12421 det The,proteins
R8055 T12421 T12422 nsubjpass proteins,analyzed
R8056 T12423 T12422 auxpass were,analyzed
R8057 T12424 T12422 prep on,analyzed
R8058 T12425 T12426 nummod 12,%
R8059 T12426 T12427 compound %,gels
R8060 T12427 T12424 pobj gels,on
R8061 T12428 T12427 compound SDS,gels
R8062 T12429 T12427 compound polyacrylamide,gels
R8063 T12430 T12422 punct .,analyzed
R8064 T12432 T12433 nmod Coomassie,staining
R8065 T12433 T12435 nsubj staining,verified
R8066 T12434 T12433 amod blue,staining
R8067 T12436 T12437 amod equal,loading
R8068 T12437 T12435 dobj loading,verified
R8069 T12438 T12437 prep of,loading
R8070 T12439 T12440 compound GST,protein
R8071 T12440 T12438 pobj protein,of
R8072 T12441 T12440 compound fusion,protein
R8073 T12442 T12435 punct .,verified
R8074 T12444 T12445 amod Dried,gels
R8075 T12445 T12446 nsubjpass gels,autoradiographed
R8076 T12447 T12446 auxpass were,autoradiographed
R8077 T12448 T12446 punct .,autoradiographed

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12058 4-9 PR_EXT:000016007 denotes TACC2
T12059 10-14 SO_EXT:cDNA denotes cDNA
T12060 19-25 SO_EXT:sequence_cloning_process denotes cloned
T12061 31-34 GO_PR_EXT:glutathione_S_transferase denotes GST
T12062 35-41 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T12063 42-48 SO_EXT:0000440 denotes vector
T12064 102-105 GO_PR_EXT:glutathione_S_transferase denotes GST
T12065 110-113 GO_PR_EXT:glutathione_S_transferase denotes GST
T12066 114-119 PR_EXT:000016007 denotes TACC2
T12067 120-128 CHEBI_PR_EXT:protein denotes proteins
T12068 134-143 GO:0010467 denotes expressed
T12069 147-154 NCBITaxon:562 denotes E. coli
T12070 184-188 CHEBI:61448 denotes IPTG
T12071 215-220 CL_GO_EXT:cell denotes Cells
T12072 277-281 CHEBI:9754 denotes Tris
T12073 282-285 CHEBI:17883 denotes HCl
T12074 301-305 CHEBI:26710 denotes NaCl
T12075 312-316 CHEBI_EXT:EDTA denotes EDTA
T12076 324-342 CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor denotes Protease inhibitor
T12077 369-374 CL_GO_EXT:cell denotes cells
T12078 380-385 GO:0019835 denotes lysed
T12079 493-502 GO_EXT:biological_movement_or_translocation_process denotes mobilized
T12080 506-517 CHEBI:16856 denotes glutathione
T12081 551-555 CHEBI:9754 denotes Tris
T12082 556-559 CHEBI:17883 denotes HCl
T12083 575-579 CHEBI:26710 denotes NaCl
T12084 586-590 CHEBI_EXT:EDTA denotes EDTA
T12085 605-609 SO_EXT:cDNA denotes cDNA
T12086 614-620 SO_EXT:sequence_cloning_process denotes cloned
T12087 673-680 CHEBI_PR_EXT:protein denotes protein
T12088 714-727 GO_EXT:transcription denotes transcription
T12089 728-739 GO:0006412 denotes translation
T12090 765-777 CHEBI_EXT:radiolabel_process denotes radiolabeled
T12091 783-786 CHEBI:37983 denotes 35S
T12092 787-797 CHEBI_SO_EXT:methionine denotes methionine
T12093 859-869 GO:0006412 denotes translated
T12094 876-883 CHEBI_PR_EXT:protein denotes protein
T12095 901-905 CHEBI:9754 denotes Tris
T12096 906-909 CHEBI:17883 denotes HCl
T12097 925-929 CHEBI:26710 denotes NaCl
T12098 936-940 CHEBI_EXT:EDTA denotes EDTA
T12099 975-984 GO_EXT:biological_movement_or_translocation_process denotes mobilized
T12100 985-988 GO_PR_EXT:glutathione_S_transferase denotes GST
T12101 989-994 PR_EXT:000016007 denotes TACC2
T12102 998-1001 GO_PR_EXT:glutathione_S_transferase denotes GST
T12103 1016-1021 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes bound
T12104 1074-1078 CHEBI:9754 denotes Tris
T12105 1079-1082 CHEBI:17883 denotes HCl
T12106 1098-1102 CHEBI:26710 denotes NaCl
T12107 1109-1113 CHEBI_EXT:EDTA denotes EDTA
T12108 1121-1126 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes Bound
T12109 1127-1135 CHEBI_PR_EXT:protein denotes proteins
T12110 1205-1211 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T12111 1220-1224 CHEBI:9754 denotes Tris
T12112 1225-1228 CHEBI:17883 denotes HCl
T12113 1251-1262 CHEBI:16856 denotes glutathione
T12114 1269-1277 CHEBI_PR_EXT:protein denotes proteins
T12115 1299-1302 CHEBI:8984 denotes SDS
T12116 1303-1317 CHEBI_EXT:polyacrylamide denotes polyacrylamide
T12117 1374-1377 GO_PR_EXT:glutathione_S_transferase denotes GST
T12118 1378-1384 SO_EXT:sequence_fusion_entity_or_process denotes fusion
T12119 1385-1392 CHEBI_PR_EXT:protein denotes protein

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12029 4-9 PR:000016007 denotes TACC2
T12030 42-48 SO:0000440 denotes vector
T12031 114-119 PR:000016007 denotes TACC2
T12032 134-143 GO:0010467 denotes expressed
T12033 147-154 NCBITaxon:562 denotes E. coli
T12034 184-188 CHEBI:61448 denotes IPTG
T12035 277-281 CHEBI:9754 denotes Tris
T12036 282-285 CHEBI:17883 denotes HCl
T12037 301-305 CHEBI:26710 denotes NaCl
T12038 380-385 GO:0019835 denotes lysed
T12039 506-517 CHEBI:16856 denotes glutathione
T12040 551-555 CHEBI:9754 denotes Tris
T12041 556-559 CHEBI:17883 denotes HCl
T12042 575-579 CHEBI:26710 denotes NaCl
T12043 728-739 GO:0006412 denotes translation
T12044 783-786 CHEBI:37983 denotes 35S
T12045 859-869 GO:0006412 denotes translated
T12046 901-905 CHEBI:9754 denotes Tris
T12047 906-909 CHEBI:17883 denotes HCl
T12048 925-929 CHEBI:26710 denotes NaCl
T12049 989-994 PR:000016007 denotes TACC2
T12050 1074-1078 CHEBI:9754 denotes Tris
T12051 1079-1082 CHEBI:17883 denotes HCl
T12052 1098-1102 CHEBI:26710 denotes NaCl
T12053 1220-1224 CHEBI:9754 denotes Tris
T12054 1225-1228 CHEBI:17883 denotes HCl
T12055 1251-1262 CHEBI:16856 denotes glutathione
T12056 1299-1302 CHEBI:8984 denotes SDS