PMC:441373 / 43286-43672 JSONTXT

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    craft-sa-dev

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For cloning the mouse Tacc1X cDNA, the primers used were based upon the genomic sequence reported, and the sequence of the IMAGE cDNA clone 4933429K08: T1XF (5'-ccatgttcagtcattggcaggtc-3'), T1XF2 (5'-ctgcagaaccaacagttcaag-3'), T1XR1 (5'-agatctgtgacatcacagctc-3'), T1XR2 (5'-ctcgagtcagttagtcttatccagctt-3'), BB617F (5'-accaccaacttgagtacctg-3') and BB617R (5'-gtatcttgaactgttggttctg-3')."}

    craft-ca-core-ex-dev

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    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T10352","span":{"begin":17,"end":22},"obj":"NCBITaxon:10088"},{"id":"T10353","span":{"begin":40,"end":47},"obj":"SO:0000112"},{"id":"T10354","span":{"begin":73,"end":80},"obj":"SO:0001026"},{"id":"T10355","span":{"begin":130,"end":140},"obj":"SO:0000317"}],"text":" For cloning the mouse Tacc1X cDNA, the primers used were based upon the genomic sequence reported, and the sequence of the IMAGE cDNA clone 4933429K08: T1XF (5'-ccatgttcagtcattggcaggtc-3'), T1XF2 (5'-ctgcagaaccaacagttcaag-3'), T1XR1 (5'-agatctgtgacatcacagctc-3'), T1XR2 (5'-ctcgagtcagttagtcttatccagctt-3'), BB617F (5'-accaccaacttgagtacctg-3') and BB617R (5'-gtatcttgaactgttggttctg-3')."}