PMC:4385177 / 17405-17898 JSONTXT

{"project":"2_test","denotations":[{"id":"25799108-23340218-2046593","span":{"begin":155,"end":157},"obj":"23340218"}],"text":"For primary cortical cultures, Wistar WT rat pups were culled between postnatal days 1 and 3, and a primary co-culture was prepared as described elsewhere.22 Cerebral hemispheres were trypsinized and resuspended in 2 ml of warm complete Neurobasal A medium, and the cell suspension was plated on poly-L-lysine-coated coverslips. The cultures were incubated at 37°C in a humidified incubator with 5% CO2 in the air for 3–4 hr, and then 2 ml of pre-warmed complete Neurobasal A medium was added."}