PMC:4349302 / 9552-9863 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4349302","sourcedb":"PMC","sourceid":"4349302","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4349302","text":"Whole cell extracts were obtained by solubilizing cells in lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM ethylene glycol tetraacetic acid (EGTA), plus Complete protease inhibitors and PhosSTOP (Roche Diagnostics)). Lysates were clarified by centrifugation at 16,000g for 15 min.","tracks":[]}