PMC:4307275 / 7197-8474 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4307275","sourcedb":"PMC","sourceid":"4307275","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4307275","text":"Subcellular localization and expression efficiency of sGFPs in rice protoplasts. (A) Three kinds of sGFP chimera were cloned into a pB2GW7 vector to construct sGFP, K-sGFP, and KR-sGFP for use in PEG-mediated transfection into rice protoplasts. Kz: Kozak sequence; rRTp: a transit peptide of rice RuBisCo small subunit; (B) Individual and merged images of GFP, chlorophyll autofluorescence, and visible protoplasts. C: chloroplast; V: vacuole. The images were acquired at 630× magnification using a confocal microscope. The yellow signals are the merged image of the green fluorescent signals from the sGFP proteins; the red fluorescent signals from chlorophyll in the chloroplasts. The PEG-transformed protoplasts with no plasmid were used as a mock sample. The yellow scale bars on the images show the mean intensity ratio of the green fluorescence signals (for 105 pixels), analyzed by a Histogram tool, and the white scale bars mean 5 μm and (C) The expression efficiency of proteins was compared using western blot analysis. Polyclonal rabbit antibody against sGFP was used at a titer of 1:5000 and the image of PAGE gel was used to show relative quantities of the loaded proteins. The graph shows the relative band intensities that were quantified using LAS4000 software.","tracks":[]}