PMC:4307275 / 28632-29039 JSONTXT

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{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4307275","sourcedb":"PMC","sourceid":"4307275","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4307275","text":"The dehulled seeds of rice (Oryza sativa L. Japonica cv. “Ilmi”) were sterilized by treatment once in 70% ethanol for 1 min and twice in 2% sodium hypochlorite for 20 min and then planted on Murashige and Skoog (MS) agar medium after washing five times with distilled water. The rice seedlings were grown in the dark for 10 days, and then illuminated with white light for 20 h prior to protoplast isolation.","tracks":[]}