PMC:4307275 / 26605-27502 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4307275","sourcedb":"PMC","sourceid":"4307275","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4307275","text":"Taken together, our results show that the chloroplast targeting of sGFP caused an increase in the population with high fluorescence intensity (\u003e104) to result in an increase in the average fluorescence intensity; the increase in the population with the fluorescence intensity above the threshold value increased the transfection ratio of FCA. Our strategy using chloroplast targeting is very useful to utilize the advantage of FCA or FACS using protoplasts of green tissues. Also, while a previous study reported DNA-usage in the range of 20–100 μg for transfection [9], our results suggest that only 5–10 μg DNA was enough for FCA. This reduction of DNA-usage on FCA using protoplasts will also be useful for saving time and effort. Likewise, the usefulness of our strategy using rRTp-sGFP should further be investigated with regard to other non-green tissues, such as roots without chloroplasts.","tracks":[{"project":"2_test","denotations":[{"id":"25561231-11746085-50799917","span":{"begin":567,"end":568},"obj":"11746085"},{"id":"T85257","span":{"begin":567,"end":568},"obj":"11746085"}],"attributes":[{"subj":"25561231-11746085-50799917","pred":"source","obj":"2_test"},{"subj":"T85257","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#d2ec93","default":true}]}]}}