PMC:4307275 / 22769-23919 JSONTXT

{"project":"2_test","denotations":[{"id":"25561231-9759496-50799912","span":{"begin":332,"end":334},"obj":"9759496"},{"id":"T67438","span":{"begin":332,"end":334},"obj":"9759496"}],"text":"Previous study has mentioned that the fluorescence intensity of the GFP on non-imaging detection system (e.g., a fluorometry using cuvets or microtiter plates, or a flow cytometry) tends to be inefficiently measured, compared to it on the imaging detection system of GFPs (e.g., a confocal microscopy or a fluorescence microscopy) [24]. This report could partly explain our finding that the fluorescence intensity of K-sGFP on FCA was measured much lower than in microscopic imaging analysis. At this point, it is worth noting that an average of 20–30 chloroplasts in rice protoplasts are mostly positioned underneath cellular membrane layers. Cautiously, we could hypothesize that those characteristics of chloroplasts might help to concentrate stable fluorophores and reduce some effects of refractive index and it might consequentially overcome some problems caused by non-imaging detection methods such as FCA. Since the question of how the chloroplast localization of sGFP causes the increase of fluorescence intensity on FCA remains, it should be further analyzed for improving the fluorescence intensity of GFPs on FCA using plant protoplasts."}