PMC:4301621 / 5868-6496 JSONTXT

{"project":"2_test","denotations":[{"id":"25582171-22174787-14905476","span":{"begin":127,"end":128},"obj":"22174787"},{"id":"25582171-21336613-14905477","span":{"begin":270,"end":272},"obj":"21336613"}],"text":"In this study, a genome-scale metabolic model (iCY1106) of M. alpina ATCC 32222 was reconstructed based on sequencing results [2]. Using this model, we first identified essential genes and reactions in fermentation medium containing sodium nitrate as a nitrogen source [23]. The de novo synthesis pathways of PUFAs such as ARA and eicosapentaenoic acid (EPA) were subsequently resolved. The roles of acetyl-CoA and NADPH in the regulation of PUFA biosynthesis were probed, and important genes and reactions were systematically analyzed using FBA, flux variability analysis (FVA), and minimization of metabolic adjustment (MOMA)."}