PMC:4301621 / 26508-27735 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4301621","sourcedb":"PMC","sourceid":"4301621","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4301621","text":"During PUFA biosynthesis, NADPH is a necessary cofactor for de novo fatty acid synthesis involving desaturation and elongation. In model iCY1106, there were 172 reactions involving NADPH (Additional file 6). With ARA production fixed at 0.059 mmol gDW−1 h−1 (μ = 0.06 h−1) and 0.128 mmol gDW−1 h−1 (μ = 0.03 h−1), the flux of NADPH reactions were investigated. In total, 53 NADPH reactions exhibited flux changes of \u003e 10−6 mmol gDW−1 h−1 (Figure 5).\nFigure 5 Changes of reactions involving NADPH at different ARA levels. (Different colors represent different changes on NADPH flux. Flux obviously changed reactions are listed. R2: D-glucose 6-phosphate + NADP ⬄ 6-phospho-D-glucono-1,5-lactone + NADPH + H, R3: 6-phospho-D-gluconate + NADP - \u003e D-ribulose 5-phosphate + CO2 + NADPH + H, R4: (S)-malate + NADP - \u003e pyruvate + CO2 + NADPH, R5: L-glutamate 5-semialdehyde + NAD P+ H2O - \u003e L-glutamate + NADPH + H, R6: 1-pyrroline-5-carboxylate + NADPH + H ⬄ L-proline + NADP, R16: Nitrite + 3 NADPH + 5 H - \u003e Ammonia + 3 NADP + 2 H2O, R51: N-acetyl-L-glutamyl 5-phosphate + NADPH + H ⬄ N-acetyl-L-glutamate 5-semialdehyde + NADP + Phosphate, R53: (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H ⬄ (R)-mevalonate + CoA + 2 NADP).","divisions":[{"label":"label","span":{"begin":450,"end":458}}],"tracks":[]}