PMC:4301621 / 25605-26507 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4301621","sourcedb":"PMC","sourceid":"4301621","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4301621","text":"During the growth stages, the flux of acetyl-CoA used to synthesize malonyl-CoA, catalyzed by acetyl-CoA carboxylase (ACC, EC 6.4.1.2), was 0.79 mmol gDW−1 h−1. In contrast during the ARA production stage, the corresponding flux was 1.43 mmol gDW−1 h−1 (81.0%). ACC catalyzes the first committal step of the fatty acid biosynthetic pathway, and should be overexpressed to maximize ARA production [39]. Glutamate has been shown to activate ACC, and adding glutamate to the culture medium led to an increase in total lipid (21.81%) and ARA yield (66.07%) [40]. As the M. alpina growth rate in batch culture ranged from 0.06 h−1 to 0.19 h-1 [1,7,8,41,42], the glutamate uptake rates used for the in silico analysis should be set between 0 and 1.8 mmol gDW−1 h−1. When the glutamate uptake rate was increased, ARA production increased from 0.128 mmol gDW−1 h−1 to 0.355 mmol gDW−1 h−1 (Additional file 5c).","tracks":[{"project":"2_test","denotations":[{"id":"25582171-10893421-14905499","span":{"begin":397,"end":399},"obj":"10893421"},{"id":"25582171-12618051-14905500","span":{"begin":554,"end":556},"obj":"12618051"},{"id":"25582171-17439233-14905501","span":{"begin":639,"end":640},"obj":"17439233"}],"attributes":[{"subj":"25582171-10893421-14905499","pred":"source","obj":"2_test"},{"subj":"25582171-12618051-14905500","pred":"source","obj":"2_test"},{"subj":"25582171-17439233-14905501","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93af","default":true}]}]}}