PMC:4301621 / 10882-11547 JSONTXT

{"project":"2_test","denotations":[{"id":"25582171-21336613-14905483","span":{"begin":89,"end":91},"obj":"21336613"}],"text":"When cultured on minimal growth medium (MG) containing glucose, KH2PO4, MgSO4 and NaNO3 [23], 86 M. alpina genes (7.78% of the total) were identified as essential for growth using the FBA method (Additional file 3). In contrast, on yeast extract (YE) medium (based on MG but including all 20 regular amino acids) only 49 genes were identified as essential. On MG medium, over half of the essential genes were involved in amino acid (36.05%) and nucleotide metabolism (23.26%; Figure 2). However on YE medium, most of the essential genes were associated with nucleotide metabolism (38.78%).\nFigure 2 Essential genes identified by MG and YE medium in model i CY1106."}