PMC:4236617 / 6034-7140 JSONTXT

{"project":"2_test","denotations":[{"id":"25070625-2023259-12189960","span":{"begin":599,"end":601},"obj":"2023259"}],"text":"Bacterial strains and culture conditions\nThe bacterial strains used in this study are listed in Table 1. Lactobacillus strains were cultivated anaerobically in MRS-broth (Difco, BD, Franklin Lakes, New Jersey) at +37°C. Enterotoxigenic Escherichia coli (ETEC) was cultured in Luria-Bertani (LB) broth (Difco, BD) with agitation at +37°C in the experiments assessing the effect of Lactobacillus strains on ETEC adherence. The E. coli strains used for the cloning and expression of Slp-encoding genes were cultivated with agitation in LB broth, or in the heterologous gene expression, in M9ZB medium [30] at +37°C, with kanamycin (30 μg/ml) being added when appropriate. In the pathogen inhibition assays, all the pathogens were cultivated in tryptic soy agar (TSA) plates (Difco) and subcultured in tryptic soy broth (TSB, Difco) at +37°C with agitation.\nTable 1 Strains used in this study amolecular weight of mature protein bnot tested for adherence cnot present dculture collection of the Department of Veterinary Biosciences/Veterinary Microbiology and Epidemiology, University of Helsinki, Finland.\n\n"}

{"project":"MicrobeTaxon","denotations":[{"id":"T58","span":{"begin":0,"end":9},"obj":"2"},{"id":"T59","span":{"begin":45,"end":54},"obj":"2"},{"id":"T60","span":{"begin":105,"end":118},"obj":"1578"},{"id":"T61","span":{"begin":380,"end":393},"obj":"1578"},{"id":"T62","span":{"begin":236,"end":252},"obj":"562"},{"id":"T63","span":{"begin":425,"end":432},"obj":"562"},{"id":"T64","span":{"begin":254,"end":258},"obj":"562"},{"id":"T65","span":{"begin":405,"end":409},"obj":"562"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"Bacterial strains and culture conditions\nThe bacterial strains used in this study are listed in Table 1. Lactobacillus strains were cultivated anaerobically in MRS-broth (Difco, BD, Franklin Lakes, New Jersey) at +37°C. Enterotoxigenic Escherichia coli (ETEC) was cultured in Luria-Bertani (LB) broth (Difco, BD) with agitation at +37°C in the experiments assessing the effect of Lactobacillus strains on ETEC adherence. The E. coli strains used for the cloning and expression of Slp-encoding genes were cultivated with agitation in LB broth, or in the heterologous gene expression, in M9ZB medium [30] at +37°C, with kanamycin (30 μg/ml) being added when appropriate. In the pathogen inhibition assays, all the pathogens were cultivated in tryptic soy agar (TSA) plates (Difco) and subcultured in tryptic soy broth (TSB, Difco) at +37°C with agitation.\nTable 1 Strains used in this study amolecular weight of mature protein bnot tested for adherence cnot present dculture collection of the Department of Veterinary Biosciences/Veterinary Microbiology and Epidemiology, University of Helsinki, Finland.\n\n"}